Effects of parasitization by Scleroderma guani and injection of its venom on the expression of catalase gene in Tenebrio molitor
In order to investigate the effects of parasitization by Scleroderma guani and injection of its venom on the expression of catalase gene in Tenebrio molitor pupae.The catalase gene of T.molitor was cloned by RT-PCR.Its sequence characteristics were analyzed by bioinformatics software.Real-time fluorescence quantitative PCR was used to analyze the gene expression patterns of catalase gene.The enzyme activity of the catalase was determined with the kit.The cloned open reading frame of the catalase gene of T.Molitor was 1 620 bp in length,encoding 539 amino acids.There are catalytic amino acids(His-71,Asn-183,Tyr-393)and three conserved motifs present in the amino acid sequence encoding by this gene,proximal active site signature(FDRERIPERVVHAKGXG),NADPH binding site(XXHQXXXXFXD)and proximal heme-ligand signature(RXFXYXDXH).After parasitization by S.guani and injection of its venom,the gene expression level of catalase in pupae was significantly up-regulated,and the catalase activity in the hemolymph and fat body of the T.molitor pupae was significantly increased.The results indicate that venom of S.guani participates in regulating the expression of catalase in T.molitor.
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