Analysis of gut microbiota diversity in male and female adults of Catharsius molossus
The diversity of the gut microbiota in insects plays a very important role in the growth and development of the host with the digestion and absorption of nutrients.This study utilized 16S rRNA gene sequences to construct a clone library of gut microbiota,investigating and comparing the microbial community structures of female and male adults of Catharsius molossus.High-throughput sequencing with Illumina NovaSeq technology was employed to reveal the similarities and differences in microbial composition between the two groups.The findings revealed substantial differences in gut microbiota composition and structure between female and male adults of C.molossus.Species richness and diversity of the gut microbiota were higher in females.Both females and males shared 1253 AS Vs within their gut microbiota,classified into 20 phyla,35 classes,54 orders,134 families,and 253 genera.Specifically,the gut microbiota of females contains 1 024 ASVs,while that of males contains 410 AS Vs.While both exhibited Vagococcus as a dominant genus,the proportions differed between the two sexes.This study analyzed the diversity of gut microbiota and the differences in community structure and composition between male and female adults of C.molossus,providing theoretical support for exploring the potential functions of gut microbiota and their practical applications.