Susceptibility monitoring and detoxification enzyme activity analysis of Dasyhippus barbipes(Orthoptera:Acridoidea)to beta-cypermethrin,azadirachtin and matrine
In order to clarify the insecticide sensitivity status and metabolic resistance mechanism of Dasyhippus barbipes field population to beta-cypermethrin,azadirachtin and matrine.The resistance of the 3 d instar nymphs of D.barbipes to three insecticides was determined by drop method in Siziwang Banner(SB),Xilinhot City(XC),Zhengxiangbai Banner(BQ),Zalut Banner(ZB)and Xinbaerhu Left Banner(XB).The activities of Carboxylesterase(CarE),Glutathione-S-transferase(GST),Multifunctional oxidase(MFO)and Cytochrome P450(CYP450)in different field populations of D.barbipes were detected,and the correlation between the resistance level and the activities of different detoxification enzymes was analyzed.The results showed that the sensitivity of five D.barbipes populations to beta-cypermethrin was significantly reduced,and the resistance ratios of 3rd instar nymphs were 5.91,5.28,4.69,3.18 and 4.06 fold,respectively.The populations of Siziwang Banner,Zhengxiangbai Banner and Zarut Banner to matrine changed significantly,and the sensitivity of five D.barbipes populations in five regions to azadirachtin did not change significantly.The activities of detoxification enzymes in different field populations were significantly higher than those in Sensitive populations,and CarE,GST and CYP450 activities were significantly different from those in Sensitive populations,while MFO activity was not significantly increased.Correlation analysis showed that GST and CYP450 activities were positively correlated with azadirachtin and matrine resistance levels,with correlation coefficients of 0.672,0.533 and 0.891,0.845,respectively.CarE and GST activities were significantly positively correlated with beta-cypermethrin resistance,with correlation coefficients of 0.908 and 0.842,respectively.CYP450 activity was significantly positively correlated with beta-cypermethrin,and the correlation coefficient was 0.704.Path analysis showed that GST and CYP450 activities had positive effects on azadirachtin and matrine resistance,and CarE and MFO had indirect effects.CarE and GST showed positive and direct effects on beta-cypermethrin resistance,while CYP450 played an indirect role.The detoxifying enzymes of D.barbipes were closely related to the resistance level of insecticides.The synergistic effect of various detoxifying enzymes could reduce the toxicity of insecticides and promote the development of resistance.It provides a scientific basis for the scientific selection of insecticides and the improvement of control efficiency for grassland locust control.
万方数据
维普
