Biological characterization of gut bacterium Enterobacter amnigenus of the diamondback moth analyzed with Biolog Metabolic Phenomics
Enterobacter amnigenus is a predominant Gram-negative cultivated bacterial species in the larval gut of the diamondback moth,Plutella xylostella.The objective of the current study was to explore the biological properties of this bacterium.The ability of E.amnigenus to metabolize different sources of carbon,nitrogen,sulphur and phosphorus and its adaptability to different environments were investigated using the Biolog Phenotype MicroArray(PM).The nutritional requirements of E.amnigenus were wide.It was capable of metabolizing 38.42％of the carbon sources that we tested,98.42％of nitrogen sources,100％of sulphur sources and 100％of phosphorus sources.Organic acids and carbohydrates were the most informative utilization patterns for carbon sources of E.amnigenu,whereas diverse amino acids and peptides were the most informative utilization patterns for nitrogen.The bacterium showed 95 different biosynthetic pathways.It displayed a broad range of adaptability and metabolized in osmolytes with up to 6.5％sodium chloride,5％sodium sulphate,6％potassium chloride,20％ethylene glycol,6％sodium formate,4％urea,10％sodium lactate,50 mmol/L sodium benzoate(pH5.2),200 mmol/L sodium phosphate(pH7.0),100 mmol/L ammonium sulphate(pH8.0),100 mmol/L sodium nitrite and 100 mmol/L sodium nitrate.In comparison,it was unable to survive under the pressure of 11％to 12％sodium lactate.It also showed active metabolism at pH values ranging from 4.5 to 10.0,with an optimum pH of around 9.0.In the presence of different amino acids,E.amnigenus exhibited both decarboxylase and deaminase activity.The larval intestinal bacterium E.amnigenus showed a wide adaptability to nutritional,osmotic and pH environments.
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