Effect of insect resistant chili pepper leaves on physiological and biochemical indicators related to Spodoptera litura larvae
Identify the key reasons for the inhibition of the growth and development of Spodoptera litura larvae fed with the leaves of Chaotian pepper.Using artificial diet and leaves of cowhorn pepper as control,by comparing the changes in physiological and biochemical indicators of S.litura larvae fed with three different diets to analyze the reasons for abnormal growth and development of S.litura larvae fed with Chaotian pepper(Variety:BLTY 2)chill pepper leaves.The results showed that the growth and development rate of the larvae fed with BLTY 2 chill pepper leaves was slower than that of the larvae fed with Niujiao pepper(Variety:FXBX)chill pepper leaves and artificial diet,and the developmental time of each larval stage of FXBX chill pepper leaves fed larvae were significantly prolonged(P＜0.05).On the 1st,3rd,4th,5th,6th,and 7th days after feeding,the juvenile hormone(JH)content in the body of larvae fed with BLTY 2 chill pepper leaves was significantly higher than that of larvae fed with the other two diets(P＜0.05).On the 3rd,4th,5th,and 7th days after feeding,the ecdysone(Ecd)content in the body of larvae fed with BLTY 2 chill pepper leaves was significantly higher than that of larvae fed with the other two diets(P＜0.05).The changes in chitinase(CHT)activity in the body of tested larvae fed with three different diets showed significant differences,while the changes of cathepsin(CTS)activity in the body of S.litura larvae fed with two types of chili pepper leaves was similar and different from that of artificial diet fed larvae.The results indicated that BLTY 2 chill pepper mainly inhibits the growth and development of S.litura larvae by interfering with the content of JH and Ecd in their bodies.The results obtained in this study lay the foundation for the screening of plant secondary metabolites with insect resistant activity.
Spodoptera lituraCapsicum annuumgrowth and developmentinsect hormonesenzyme activity
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NSTL
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