Identification and expression analysis of chitinase genes in Nasonia vitripennis
Chitinase is a glycoside hydrolase that degrades chitin and participates in many important physiological processes in insects such as molting,organ development and immunity.At present,gene annotations and functions of chitinases in hymenopteran insects are still poorly known.In this study,14 chitinase genes were identified in the genome of the parasitoid wasp Nasonia vitripennis based on bioinformatics analysis,with the amino acid number between 312～2 682.Phylogenetic analysis showed that the chitinases were divided into 9 subfamilies,among which Group Ⅳ and Ⅶ subfamilies have been expanded in N.vitripennis,likely through gene tandem duplication.The results of qRT-PCR analysis revealed diverse expression patterns of chitinase genes,among which NvCht1,NvCht5,NvCht6 and NvCht7 had a peak at 1.5 d larvae,NvCht3 peaked at 5 d larvae,and NvCht8 at all larval stages.In addition,the qPCR analysis results in larval tissues showed that higher mRNA levels of NvCht4,NvCht5,NvCht10 and NvCht12 in the cuticle,NvCht7,NvCht8 and NvCht13 in the gut,NvCht9 in fat body and cuticle,and NvCht11 in the salivary gland.This study provides theoretical bases for the evolutionary analysis and functional study of chitinases in parasitoid wasps.
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