Genetic diversity analysis of the mitochondrial genome of Pieris rapae in China
Pieris rapae,is a notorious agricultural pest of cruciferous crops.To gain insight into the population genetic characteristics of P.rapae in China,the whole mitochondrial genomes of 48 P.rapae from five geographical groups in China were obtained by high-throughput sequencing,and the genetic structure and diversity among the different groups were analyzed.The results showed that there was high mitochondrial haplotype diversity among the populations,while nucleotide diversity was low in the relatively closed mountain and basin areas.The average genetic differentiation index(FsT)among populations is only 0.042,indicating a relatively low level of genetic differentiation among different geographic populations.Neither the mitochondrial evolutionary tree nor the network diagram formed a clear geographical genealogy,and there was no obvious genetic structure between the northern and southern populations,indicating gene flow between populations affected by transport of host vegetables.The Tajima's D and Fu's Fs values of the whole population were negative and significant,suggesting that P.rapae in China may have undergone a population expansion event.