Transcriptome analysis of Cordyceps javanica in response to host insect in saprophytic culture
Cordyceps javanica is a broad-spectrum insecticidal fungus with biocontrol potential against the social insect of hymenoptera,Solenopsis invicta.The aim of this study was to determine the key role of infected dead ants in refuse piles during the infection cycle,and investigate the molecular pathogenic mechanism of C.javanica in response to host component in saprobiotic condition.The mixture of mycelia and conidia which induced by freeze-dried bodies of ants culture before and after was analyzed by transcriptome sequencing.Our findings revealed that,compared with pure culture,C.javanica showed 1 912 new genes after induction,in which 379 novel genes were annotated with a putative function.A total of 242 significantly differentially expressed genes(DEGs)were identified between induced and uninduced culture.Among them,111 DEGs were up-regulated while 131 down-regulated;GO enrichment analysis indicated that metabolic process and cellular process classified as biological process,cellular anatomical entity classified as cellular component,and catalytic activity and binding classified as molecular function might play an important role when C.javanica adapted to saprobiotic condition;KEGG classification analysis showed that tryptophan metabolism,valine,leucine and isoleucine degradation,glycolysis/gluconeogenesis and glyoxylate and dicarboxylate metabolism of metabolism classification,base excision repair and protein processing in endoplasmic reticulum of genetic information processing classification,ABC transporters and MAPK signaling pathway-yeast of environmental information processing classification,autophagy-yeast and peroxisome of cellular processes were the main metabolic pathways.Eight genes were randomly selected to verify the expression trend by quantitative real-time fluorescence PCR.The qRT-PCR results showed that the expression trend was consistent with the results of transcriptome data.In this study,we found that the genes related to the amino acids and energy metabolism were active.The possible reasons is that the needs of maintenance of virulence in vertical and horizontal diffusion of C.javanica,and the use of nutrients converting to energy and intermediates assisted with limited host nutrients.
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