Fitness analysis of Eocanthecona furcellata fed on frozen-treated Tenebrio molitor pupae
Eocanthecona furcellata is a crucial natural enemy insect in agricultural and forestry systems.Its alternative prey for mass rearing,Tenebrio molitor pupae,were produced in surplus with a short storage time.To optimize the utilization of this alternative prey,this study investigated the feasibility of using frozen T.molitor pupae at-18 C as a food source for E.furcellata.The study assessed the nutrient composition after various freezing treatment periods,calculated the nutrient intake of E.furcellata,and analyzed life table parameters in comparison to those fed with live larvae and pupae of T.molitor.Results indicated that freezing T.molitor pupae at-18 ℃ for more than 30 d significantly reduced the protein and fat content.E.furcellata population fed on frozen pupae did not show significant differences compared to those fed on live larvae and pupae,except for a longer preadult period.Populations fed on frozen pupae had a higher net reproductive rate Ro(94.60 offspring),intrinsic rate of increase A(1.1111 d-1),and finite rate of increase r(0.1053 d-1)compared to those fed on T.molitor larvae,and slightly lower than those fed on live pupae.In conclusion,T.molitor pupae frozen at-18℃ for 7~14 days maintained their high nutritional value.When fed on frozen-treated pupae,E.furcellata populations exhibited excellent feeding capacity and reproductive adaptability.Therefore,T.molitor pupae frozen under appropriate conditions could be beneficial for rearing E.furcellata.This study provides foundational knowledge for the mass production and standardized rearing of the natural enemy insect E.furcellata.