Effects of different host plants on the growth,development and gut bacterial community of Rhoptroceros cyatheae larvae
To clarify the structural composition and diversity of the gut bacteria of Rhoptroceros cyatheae feeding on Alsophila spinulosa and Gymnosphaera metteniana var.subglabra,and the average developmental epoch on the two host plants.The Illumina HiSeq technology was used to sequence and analyze the V3～V4 regions of 16S rDNA of the gut bacteria of R.cyatheae larvae feeding on the two host plants,and recorded their developmental history.The results showed that:(1)The egg,mature larval and pupal stages(4.07±0.80 d,9.15±1.36 d and 3.82±1.11 d)of R.cyatheae feeding on G.metteniana var.subglabra were significantly shorter compared with those feeding on A.spinulosa(4.57±0.65 d,10.75±1.73 d and 4.73±0.86 d).There was no significant effect on the adult lifespan.(2)The larvae gut bacteria of R.cyatheae fed on A.spinulosa had been annotated into 4 phyla,6 classes,17 orders,26 families and 37 genera;the larvae gut bacteria of R.cyatheae fed on G.metteniana var.sub glabra had been annoted into 12 phyla,17 classes,32 orders,44 families and 58 genera.The Proteobacteria(83.68％±3.74％,81.18％±0.75％)was the dominant bacteria in both groups;Enterobacter(61.98％±10.64％,30.25％±1.75％)was the dominant genus in both groups.The alpha diversity analysis showed that the gut microbial community diversity of R.cyatheae larvae feeding on G.metteniana var.subglabra was slightly higher than that feeding on A.spinulosa.Beta diversity analysis showed that the gut microbial community of R.cyatheae larvae feeding on G.metteniana var.subglabra was more stable.KEGG functional prediction revealed no difference in the function of the gut bacterial community between R.cyatheae larvae feeding on the two host plants,and the gut bacteria were mainly involved in the nutrient metabolism of the host.This should be a case study for the factors that influence gut bacteria of R.cyatheae,and may provide insights into the interactions between gut bacteria and different host plants.This study provides a reference for further exploring the factors affecting the changes of gut bacteria of R.cyatheae and for subsequent studies on the interactions between gut bacteria and host plants.
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万方数据
