Cloning and expression pattern analysis of sex piptide receptor gene GmelSPR in Galleria mellonella L.
Mating is an important way for insect reproduction.Females will show a series of behaviors to protect their offspring after mating,and SPR is an important receptor gene regulating mating behavior.In this research,the complete CDS sequence of SPR gene from Galleria mellonella was amplified via RT-PCR,named GmelSPR(GenBank accession number:OR347698),based on the early transcriptome SPR gene data.Bioinformatics analysis showed that the full-length of the GmelSPR coding region was 1 263 bp,encoding 420 amino acids.Predicted molecular weight and theoretical isoelectric point were 48.70718 kDa and 8.70.The coding region included 7 putative transmembrane domains,with typical G protein-coupled receptor protein characteristics.Phylogenetic tree comparison results elucidated that the confidence of GmelSPR protein in G.mellonella was higher than that in Amyelois transitella and Plodia interpunctella.The expression of GmelSPR in the head of the unmated female and male moths and the mated female and male moths was higher than that in other tissues.Meanwhile,the expression levels of GmelSPR in unmated female and male moths were higher than those of mated female and male moths.In general,we cloned the SPR gene of G.mellonella and founded that GmelSPR was closely related to insects of Pyralidae.Expression profile analysis suggested that GmelSPR might be involved in mating behavior of G.mellonella.The results provide foundation for further study on the function of SPR gene in G.mellonella.
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