Expression characteristics of vitellogenin and juvenile hormone in honeybees during overwintering period
Aims to explore the expression characteristics of Vitellogenin(Vg)and Juvenile hormone(JH)in honeybee during overwintering period.In this experiment,different periods Hunchun black worker bees were collected in July,November,December,January and February,respectively.The concentration of Vg,JH Ⅲ and SOD of worker bees in different periods were detected by ELISA,and the expressions of Vg and JH metabolism-related genes of worker bees in different periods were detected by qRT-PCR.The results showed that the Vg concentration of Hunchun black bee was higher in overwintering period than summer reproduction period,and the Vg concentration generally increased and then decreased with overwinter drags on.The change trend of Vg was consistent with Vg concentration.The expression level of VgR was consistent with the change trend of SOD concentration;they showed higher level in overwintering period and downward trend during whole overwintering period.The JH Ⅲ concentration of Hunchun black bee in overwintering period was lower than summer reproduction period,and showed a continuous upward trend with the winter continuous.The change trend of MFE was similar to the concentration of JH Ⅲ.The expression level of JHAMT was significantly higher in overwintering period than summer reproduction period,and showed earlier increase and later decrease trend during the whole overwintering period.The results in our study showed that there was a negative correlation model between Vg and JH Ⅲ of honeybees in overwintering period,which might improved the antioxidant capacity and cold resistance of honeybees through regulating the expression of Vg and JH Ⅲ metabolic-related genes in overwintering period.It provides a new idea for the in-depth study of the molecular mechanism of cold resistance of western honeybees.
Hunchun black beevitellogeninjuvenile hormoneoverwintering period
NETL
NSTL
万方数据
