Non-targeted metabolomics-based analysis of differences in the gut metabolic profile of Spodoptera frugiperda between feeding corn and rice
To investigate the adaptability of Spodoptera frugiperda J.E.Smith on different host plants,we compared the development,survival and reproduction of S.frugiperda feeding on the leaves of corn Zea mays L.and rice Oryza sativa L.,respectively,under laboratory conditions.Also,the intestinal tract of 4th instar larvae was dissected,and metabolomics analysis was applied to investigate the differences in gut metabolic profiles of the larvae after feeding on two host plants(corn/rice),and to screen the important metabolites and metabolic pathways related to feeding on different hosts.The results showed that compared with the corn feeding treatment(control group),the generation time of rice feeding treatment(experimental group)was extended(38.05±0.35 d),the survival rate of pre-adult in the early stage decreased(0.46±0.01),and the Net reproductive rate R0(191.68±33.54),Intrinsic rate of increase r(0.125±6.77)and Finite rate of increase λ(1.133±7.65)were lower than those in the control group.In the gut metabolic profile,there were a total of 750 differential metabolites,of which 163 were up-regulated and 587 were down-regulated,and the down-regulated differential metabolites mainly included Histidine,Alpha-Linolenic acid(ALA)and Shikimic acid in the gut tract of S.frugiperda larvae that fed on rice leaves compared with feeding on corn leaves.The up-regulated metabolites include Asparaginyl-Cysteine and N-Acetylanonaine,which were involved in pathways closely related to the regulatory mechanisms of Amino acid metabolism and Lipid metabolism.These metabolites were involve in pathways such as Amino acid metabolism and Lipid metabolism.Our results provide clues to the adaptation mechanisms of S.frugiperda feeding on different hosts.
Fall armywormgut metabolichost transfersurvival rate
NETL
NSTL
万方数据
