Comparative analysis of metabolites in different resistant plants of Aquilaria smensis
Aquilaria sinensis Lour.is a nationally endangered protected plant,and the incense produced after its traumatization is a kind of precious medicinal herbs and spices,while the frequent occurrence of the Heortia vitessoides Moore pest has seriously affected the development of the incense industry.To explore the anti-insect physiological mechanism of A.sinensis,the present study was conducted to compare and analyze the physicochemical properties,volatiles,and differential metabolites of the leaves of six plants with different anthelmintic resistance by using indicator content determination,GC-MS analysis,and non-targeted metabolomics analysis.The results showed that the insect-resistant plant(A01)had the highest content of tannins(0.39 mg/g)and flavonoids(1.48 mg/g),while the lowest content of soluble proteins(4.5 mg/g)and soluble sugars(6.7 mg/g).Insect attractant content was significantly higher and repellent content was significantly lower in A.sinensis susceptible plants(S)than in the other 5 plants.The nutrients and volatile components in the leaves of the insect-resistant plants of A.sinensis differed significantly from those of the common insect-susceptible plants,with the nutrients and insect-inducing active volatile components in the leaves of the insect-resistant plants being low,while the repellent active volatile components were generally high.Differential metabolites between insect-resistant and common insect-susceptible plants focused on flavonoid and flavonol biosynthesis,phenylalanine biosynthesis,phenylalanine metabolism,tyrosine metabolism,and isoquinoline alkaloid biosynthesis pathways.The results of this study can provide theoretical guidance for determining the insect-resistant mechanism of A.sinensis and exploring new pollution-free prevention and control methods of H.vitessoides in the future.
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