Development of a rapid detection method for Wasmannia auropunctata based on enzyme-mediated duplex exponential amplification(EmDEA)
Wasmannia auropunctata is one of the most harmful invasive ants,posing severe threats to flora,fauna,ecosystems,economies,and even humans.It is a significant quarantine pest in China.To achieve rapid and precise on-site detection of the W.auropunctata,this study screened specific segments from the whole genome of the W.auropunctata through self-developed program,ensuring higher specificity.The study also selected suitable primers and RNA probes based on the enzyme-mediated duplex exponential amplification(EmDEA).The total reaction time required was less than 30 minutes,and both specificity and sensitivity were validated.Results demonstrate that this method could achieve extremely high detection sensitivity and specificity in a short time,with a minimum detection limit of approximately 4 ng.This method significantly reduces the dependence on equipment,making it simple,rapid,and specific,thereby providing technical support for grassroots detection and on-site diagnosis of the W.auropunctata.
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