Response of Hermetia illucens L.to zinc ion stress based on transcriptome sequencing technology
Hermetia illucens is a globally significant resource insect.Excessive zinc ion(Zn2+)stress can adversely affect the growth and development of its larvae.Transcriptome analysis provides insights into the molecular mechanisms underlying the impact of Zn2+stress on black hydra larvae.In this study,successive generations of Black Hydra larvae were subjected to different concentrations of Zn2+(75,150,300,1 200 mg/kg)stress,and the transcriptome of 6th instar larvae from the 5th generation was sequenced and analyzed by Illumina HiSeq 4000 high-throughput sequencing platform.The results showed that Zn2+stress affected the gene expression in the fifth generation H.illucens larvae.Differentially expressed genes were observed in larvae treated with 75,150,300 and 1 200 mg/kg Zn2+,with counts ranging from 1 293/1 839 to 1 444/2 170 respectively.GO enrichment analysis indicated that differentially expressed genes primarily affected cellular processes and components such as cells and cell components;while molecular functions mainly involved catalytic activity and cell binding.KEGG enrichment differential analysis revealed that under 75 mg/kg Zn2+stress,the differentially expressed genes were predominantly enriched in immune-related reactions,immune-related signal transduction pathways,and lipid metabolism.Conversely,under 300 mg/kg Zn2+treatment,the differentially expressed genes were primarily enriched in amino acid metabolism and carbohydrate metabolism.Under 1 200 mg/kg Zn2+stress,there was a significant impact on amino acid and lipid metabolism,immune-related reactions,and growth in H.illucens larvae.Most of these enriched differential genes associated with the aforementioned processes exhibited down-regulation.Therefore,Zn2+stress exerted an influence on the expression of differential genes in H.illucens larvae;particularly at high concentrations of Zn2+,which mainly affected cell processes,catalytic activity,combined gene expression resulting mostly in down-regulation.Additionally,high concentrations of Zn2+stress disrupted the immune system as well as environment-related signal transduction pathways and lipid metabolism in H.illucens larvae.
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