Analysis of bioinformatic and expression pattern of Apis mellifera AKHR gene
In this study,molecular cloning and bioinformatic analysis of the Apis mellifera gene encoding adipokinetic hormone receptor(AKHR)were conducted,followed by determination of the expression pattern of AmAKHR in various tissues and developmental stages of worker,aiming to offer reference and foundation for further functional investigation.The CDS of AmAKHR was amplified by RT-PCR.Related software were used to predict physicochemical property,molecular characteristics and protein interaction network of AmAKHR.RT-qPCR technology was employed to detect the relative expression level of AmAKHR in different tissues and developmental stages.The results demonstrated that the length of CDS of AmAKHR was about 1 050 bp,encoding 349 amino acids.The molecular weight of AmAKHR was approximately 40.63 kDa,and the molecular formula was C1873H2939N471O484S26.AmAKHR included 39 phosphorylation modification sites,7 α-transmembrane helical structures and 8 conserved motifs,but there was no signal peptide.AmAKHR was mainly distributed in the plasma membrane.The AmAKHR and the Apis florea AKHR was clustered into one clade on the evolutionary tree.The secondary structure of AmAKHR contained 127 random coils,124 alpha helices,87 extended strands and 11 beta turns.AmAKHR formed a interaction network with 10 other proteins such as Vg and Corazonin.AmAKHR was differentially expressed in six tissues of Apis mellifera worker,including venom gland,midgut,hypopharyngenal gland,fat body,brain and antrnna.The expression level of AmAKHR was highest in fat body and lowest in midgut.AmAKHR was expressed in egg,1-day-old larva,3-day-old larva,5-day-old larva,2-day-old prepupa and 1-day-old pupa,and the expression level was continuously up-regulated as day-old increased.The results reveal that AmAKHR is a kind of hydrophobic protein and membrane protein,AmAKHR potentially interact with 10 proteins such as Vg and Corazonin,AmAKHR is likely to play a role in energy metabolism,lipolysis and metamorphosis of Apis mellifera worker.
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