Effects of sublethal concentrations chlorfenapyr on growth,development and enzyme activity of Tuta absoluta
In order to evaluate the sublethal effects of chlorfenapyr on F0 generation and progeny(F1 generation)of Tuta absoluta and to explore the response of detoxification enzymes and protective enzymes in F0 and F1 of T.absoluta to chlorfenapyr.This study used the leaf soaking method to determine the toxicity of chlorfenapyr to T.absoluta,and the sublethal effects of LC25 exposure to chlorfenapyr on growth and development of F0 and F1 generations of T.absoluta were analyzed by bioassay and the changes on F0 generation and progeny(F1 generation)of T.absoluta of detoxification enzymes and protective enzymes in the body after LC25 treatment were determined.The results showed that the activity of chlorfenapyr to the 2nd instar larvae of T.absoluta was high with the LC50 value of 1.33 mg/L.The number of eggs laid in the sublethal pesticide concentrations LC25 chlorfenapyr significantly reduced of F0 generation.Exposure to sublethal pesticide concentrations reduced the weight of F1 pupae,significantly prolonged the pupal period and total pre-oviposition period.In terms of detoxification enzymes,there was no significant change in carboxylesterase(CarE)activity,but glutathione S-transferase activity(GSTs)and Mixed-function oxidase(MFO)were distinctly inhibited by sublethal concentrations of chlorfenapyr on F0 generation.There was no significant change in CarE activity compared with the control,but GSTs and MFO were distinctly increased by sublethal concentrations of chlorfenapyr on F1.In terms of protective enzymes,the sublethal concentration of chlorfenapyr had no significant affect on the activities of POD and SOD on F0 generation in T.absoluta,while there was inducedeffect on the activity of CAT and the three protective enzymes CAT,POD and SOD on F0 generation.In summary,chlorfenapyr at the sublethal concentrations could significantly affect the growth and development of T.absoluta and the activity of some detoxification enzymes and protective enzymes in vivo.Therefore,this study provided valuable information for promoting the rational use of chlorfenapyr for controlling T.absoluta.
Tuta absolutachlorfenapyrsublethal effectdetoxification enzymeprotective enzyme
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