Host adaptability analysis of Buzura suppressaria based on metabolic enzyme activity and transcriptomics
Eucalyptus spp.is one of the world's three fast-growing and high-yielding afforestation tree species,and is the most important industrial raw material species in Southern China.Buzura suppressaria is a major defoliator pest of Eucalyptus plantations,seriously affecting the normal growth of Eucalyptus and causing huge economic losses.Therefore,this study aims to explore the host adaptation of B.suppressaria to Eucalyptus based on enzyme activity determination of digestive enzymes and detoxification enzymes combined with transcriptome sequencing analysis,with the goal of providing a theoretical basis for controlling this pest.The results showed that the enzyme activities of trypsin,chymotrypsin and amylase,as well as carboxylesterase,glutathione-S-transferase and cytochrome P450 in B.suppressaria for digesting Eucalyptus after feeding on Eucalyptus leaves under different times were significantly higher than those in the control group under different time sequences.Transcriptome sequencing analysis revealed 2 003 differentially expressed genes,of which 1 148 genes were significantly up-regulated,855 genes were significantly down-regulated.At the same time,a total of 1 178 genes related to host adaptation were discovered,involving trypsin,chymotrypsin,amylase,CarE,GST and P450 genes.The digestive enzymes and detoxification enzymes and their related DEGs play an important role in the digestive and detoxification metabolism of B.suppressaria during its adaptation to its host Eucalyptus.This study provides a theoretical basis for systematically elucidating the mechanism by which the major leaf-eating pest B.suppressaria overcomes the defensive chemical substances of the host eucalyptus and causes a catastrophic outbreak.
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