Cloning of Cu/Zn-SOD1,Cu/Zn-SOD2 and Fe/Mn-SOD in Liposcelis bostrychophila(Psocoptera:Liposcelididae)and their response to high and low temperature stresses
Cloning of Cu/Zn-SOD1,Cu/Zn-SOD2 and Fe/Mn-SOD in Liposcelis bostrychophila(Psocoptera:Liposcelididae)and their response to high and low temperature stresses
[Aim]To reveal the role of the superoxide dismutase genes in Liposcelis bostrychophila in response to high and low temperature stresses.[Methods]The cDNAs of three superoxide dismutase genes Cu/Zn-SOD1,Cu/Zn-SOD2 and Fe/Mn-SOD of L.bostrychophila were cloned by RT-PCR,and their sequence characteristics were analyzed by bioinformatics methods.RT-qPCR was used to detect the relative expression levels of Cu/Zn-SOD1,Cu/Zn-SOD2 and Fe/Mn-SOD in adults at 0,1 and 2 h under high temperature(42 ℃)and low temperature(4℃)stresses.[Results]LbCu/Zn-SOD1,LbCu/Zn-SOD2 and LbFe/Mn-SOD(GenBank accession numbers are OQ938782,OQ938783 and OQ938784,respectively)of L.bostrychophila were cloned,with the open reading frames(ORFs)of 465,630 and 636 bp in length,encoding 154,209 and 211 amino acids with the relative molecular weights of 15.85,22.33 and 23.72 kD,and the isoelectric points of 6.17,7.68 and 6.79,respectively.LbCu/Zn-SOD1 and LbCu/Zn-SOD2 had one Cu/Zn superoxide dismutase signature and two Cu/Zn superoxide dismutase signatures,respectively.LbFe/Mn-SOD had one Fe/Mn superoxide dismutase signature.The results of phylogenetic analysis showed that Cu/Zn-SOD1,Cu/Zn-SOD2 and Fe/Mn-SOD were highly conserved in insects.The expression of LbCu/Zn-SOD1,LbCu/Zn-SOD2 and LbFe/Mn-SOD in adults of L.bostrychophila was induced by high temperature 42 ℃ stress,and the expression levels of LbCu/Zn-SOD1 and LbCu/Zn-SOD2 in adults of L.bostrychophila were significantly higher than that of the control at 1 and 2 h.The expression level of LbFe/Mn-SOD in adults of L.bostrychophila was significantly lower than that of the control at 1 h and significantly higher than that of the control at 2 h under 42 ℃ stress.There was no significant difference in the expression levels of LbCu/Zn-SOD1 and LbCu/Zn-SOD2 in adults of L.bostrychophila at 1 h,while that of LbFe/Mn-SOD decreased significantly at 1 h and those of LbCu/Zn-SOD1,LbCu/Zn-SOD2 and LbFe/Mn-SOD increased significantly at 2 h under 4 ℃ stress as compared with that of the control.[Conclusion]The superoxide dismutase genes LbCu/Zn-SOD1,LbCu/Zn-SOD2 and LbFe/Mn-SOD are involved in the tolerance of L.bostrychophila to extreme temperature stress.
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