胰岛素对昆虫病原线虫卡森斯氏线虫0657L生长发育及其共生细菌伯氏致病杆菌0657L的影响

扫码查看

原文链接

万方数据
维普

中文摘要：[目的]为探讨胰岛素对昆虫病原线虫卡森斯氏线虫Steinernema kraussei 0657L生长发育及其共生细菌的影响.[方法]吸取1.5 mL 3 000头/mL的卡森斯氏线虫0657L悬浮液侵染大蜡螟Galleria mellonella 3龄幼虫,25℃培养24 h后分离卡森斯氏线虫0657L的共生细菌伯氏致病杆菌Xenorhabdus bovenii 0657L;分别用1:500胰岛素和1∶5 000胰岛素处理伯氏致病杆菌0657L后饲喂卡森斯氏线虫0657L,借助体视显微镜观测伯氏致病杆菌0657L在胰岛素处理1～6 d时的菌落直径并计算生长速率,计算卡森斯氏线虫0657L寿命、存活率、每30 s头部摆动次数和每30 s吞咽次数,评价其平均寿命、运动能力及吞咽能力.[结果]与空白对照组相比,1∶5 000胰岛素和1∶500胰岛素处理组伯氏致病杆菌0657L的菌落直径在对数生长期(处理2 d时)可分别增大77.2％和54.0％,生长速率可由空白对照组的68.0 μm/d分别提高至120.4和104.7 μm/d;卡森斯氏线虫0657L寿命分别延长27.3％和45.5％;卡森斯氏线虫0657L 3龄幼虫每30 s头部摆动次数分别提高56.3％和74.7％,3和4龄幼虫及成虫每30 s吞咽次数分别提高50.9％和65.5％,26.8％和35.2％及23.7％和21.1％.[结论]胰岛素可以显著促进卡森斯氏线虫0657L共生细菌伯氏致病杆菌0657L单菌落的生长和提高其生长速率,能够通过伯氏致病杆菌0657L间接延长卡森斯氏线虫0657L的寿命,提高昆虫病原线虫的运动和吞咽能力.本研究结果可以为延长昆虫病原线虫商品货架期提供科学依据,为昆虫病原线虫的高效生物防治提供理论基础.

外文标题：Effects of insulin on the growth and development of the entomopathetic nematode Steinernema kraussei 0657L and its symbiotic bacterium Xenorhabdus bovenii 0657L

外文摘要：[Aim]To investigate the effects of insulin on the growth and development of the entomopathogenic nematode Steinernema kraussei 0657L and its symbiotic bacterium.[Methods]The 3rd instar larvae of Galleria mellonella were infected with 1.5 mL of 3 000/mL S.kraussei 0657 L suspension,the symbiotic bacterium Xenorhabdus bovenii 0657L was isolated from S.kraussei 0657 L after 24 h culture at 25 ℃.S.kraussei 0657L was fed with X.bovenii 0657L treated with 1∶500 and 1∶5 000 insulin,respectively,the colonial diameter and growth rate of X.bovenii 0657L were measured by stereomicroscope at 1-6 d after insulin treatment,the lifespan,survival rate,number of head swing per 30 s and number of swallows per 30 s of S.kraussei 0657 L were calculated to evaluate its average lifespan,locomotion and swallowing ability.[Results]The colonial diameters of X.bovenii 0657L in 1:5 000 insulin and 1∶500 insulin treatment groups were increased by 77.2％and 54.0％,respectively,during the logarithmic growth period(at 2 d after treatment)as compared with that of the blank control group.The growth rates of X.bovenii 0657 L in 1:5 000 insulin and 1:500 insulin treatment groups were increased from 68.0 μm/d of the blank control group to 120.4 and 104.7 μm/d,respectively.The lifespans of S.kraussei 0657 L in 1:5 000 insulin and 1:500 insulin treatment groups were increased by 27.3％and 45.5％,respectively,as compared with that of the blank control group.The numbers of head swing per 30 s of the 3rd instar larvae of S.kraussei 0657L in 1∶5 000 insulin and 1:500 insulin treatment groups were increased by 56.3％and 74.7％,respectively,as compared with that of the blank control group.The numbers of swallows per 30 s of the 3rd and 4th instar larvae and adults of S.kraussei 0657L in 1∶5 000 insulin and 1∶500 insulin treatment groups were increased by 50.9％and 65.5％,26.8％and 35.2％,23.7％and 21.1％,respectively,as compared with that of the blank control group.[Conclusion]Insulin can significantly promote the growth and increase the growth rate of single colony of the symbiotic bacterium X.bovenii 0657L of S.kraussei 0657L,which can prolong the lifespan of S.kraussei 0657 L and improve the locomotion and swallowing ability of entomopathogenic nematode indirectly.The results of this study can provide a scientific basis for extending the shelf life of entomopathogenic nematodes and provide a theoretical basis for efficient biological control of entomopathogenic nematodes.

外文关键词：

Steinernema krausseiXenorhabdus boveniiinsulinentomopathogenic nematodessymbiotic bacteriagrowth and developmentlifespanlocomotionswallow

作者：

李茜童、陈红、王兴铎、钱秀娟

展开 >

作者单位：

甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州 730070

关键词：

卡森斯氏线虫伯氏致病杆菌胰岛素昆虫病原线虫共生细菌生长发育寿命运动吞咽

基金：

国家自然科学基金项目甘肃省科技计划项目西藏自治区科技计划项目

项目编号：

3196055921JR7RA819XZ202301ZY0019N

出版年：

2024

DOI：

10.16380/j.kcxb.2024.01.005

昆虫学报

中国科学院动物研究所,中国昆虫学会

昆虫学报

CSTPCD北大核心

影响因子：0.756

ISSN：0454-6296

年,卷(期)：2024.67(1)

参考文献量48