Symbiotic bacterial transfer in a mixed culture of Nilaparvata lugens and Sogatella furcifera(Hemiptera:Delphacidae)
[Aim]To investigate whether the interspecific interaction of Nilaparvata lugens and Sogatella furcifera leads to changes in their symbiotic bacteria.[Methods]N.lugens and S.furcifera were reared in either pure-or mixed-species cultures,and the diversity of the symbiotic bacteria of these populations was analyzed by 16S rRNA gene sequencing.[Results]The structure of the symbiotic bacterial communities of both N.lugens and S.furcifera in mixed-species culture changed significantly as compared with that of both N.lugens and S.furcifera in pure-species culture,and the symbiotic bacterial community structural convergence of N.lugens and S.furcifera in mixed-species culture was observed.The diversity of the symbiotic bacteria of N.lugens in mixed-species culture was significantly enhanced as compared with that in pure-species culture.A total of 133 amplicon sequence variants(ASVs)in N.lugens and 143 ASVs in S.furcifera were defined as potential horizontally transferred species.[Conclusion]Interspecific interaction between N.lugens and S.furcifera alters the symbiotic bacterial communities of the hosts,providing a new perspective of symbiotic bacteria for studying the interspecific interaction between planthoppers.
万方数据
维普
