分离提取纯净完整的RNA对于分子克隆实验是很重要的,而且是进行基因表达分析的基础。本实验通过总RNA纯化试剂盒(V-gene Total RNA Purification Kit)从蚯蚓体内提取蚯蚓纤溶酶总RNA,为下一步进行RT-PCR等分子克隆实验打下了基础。
Total RNA Extraction and Its Electrophoresis Analysis of Earthworm Fibrinolytic Enzyme
Total RNA isolation and purification is a very important step in molecular cloning experiments, and it is also the basis of gene expression and analysis .The quanity and quality of the total RNA extracted from the earthworm fibrinolytic enzyme by V-gene T