Analysis of Endophytic Bacterial Diversity of Three Polygonatum Species Based on High-throughput Sequencing Technology
The 16S rRNA genes of rhizome endophytic bacteria from three Polygonatum species(P.kingianum Coll.et Hemsl,P.cyrtonema Hua,and P.sibiricum Delar.ex Redoute)were determined by high-throughput sequencing,aiming to investigate the diversity and community structure among different Polygonatum species.A total of 179 operational taxonomic units(OTUs)were identified in the rhizomes of the three Polygonatum species,with 74 OTUs obtained from P.kingianum Coll.et Hemsl,178 OTUs from P.cyrtonema Hua,and 18 OTUs from P.sibiricum Delar.ex Redoute,respectively.The total common number of OTUs in the three species was 16,accounting for 8.93%of the total.OTUs can be classified into 11 phyla,14 classes,38 orders,52 families,87 genera,and 79 species,excluding the unclassified taxa.At the phylum level,Proteobacteria emerged as the dominant phylum in P.cyrtonema Hua,and P.sibiricum Delar.ex Redoute,whereas Cyanobacteria dominated in P.kingianum Coll.et Hemsl.In terms of genus composition,the dominant bacteria genera of P.cyrtonema Hua,P.kingianum Coll.et Hemsl and P.sibiricum Delar.ex Redoute were completely different,Klebsiella(27.74%),Delftia(80.28%),and Pseudomonas(48.99%)were identified as the dominant bacterial genera,respectively.Functional prediction analysis revealed that the endophytic bacteria derived from the rhizomes of the three Polygonatum species exhibit slight variations in their functions,with the higher abundance bacteria having mainly metabolism-related functions.Overall,the rhizomes of the three Polygonatum species harbor a large array of endophytic bacteria,with distinct dissimilarities among them.The results may lay a foundation for further exploitation of endophytic bacteria and subsequent development and application of functional strains with Polygonatum species.
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