Study on Chemical Components and Anti-inflammatory Effects of Water Decoction of Spuriopimpinella brachycarpa
To identify the chemical components in the water decoction of Spuriopimpinella brachycarpa and evaluate its anti-inflammatory effects,UHPLC-Q-Exactive Orbitrap-MS technology was used for analysis.Meanwhile,the anti-inflammatory effect of the water decoction of Spuriopimpinella brachycarpa was studied via LPS-induced zebrafish bacterial inflammation model and immunofluorescence staining technology.Fourteen chemical components were identified,including 3 flavonoids(apigenin,diosmin,eugenol-3-glucose)and 11 phenolic acids(neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid,etc.).The anti-inflammatory study showed that LPS-induced inflammation significantly increased the number of neutrophils,but after drug treatment,the number of neutrophils was significantly lower than that of the untreated model group,manifesting the certain anti-inflammatory effect of the water decoction of Spuriopimpinella brachycarpa.In addition,the IC50 calculation results showed that the IC50 of the water decoction of Spuriopimpinella brachycarpa for zebrafish was about 110 μg/mL.These results provide a theoretical basis for the pharmacological action and clinical application of Spuriopimpinella brachycarpa,and render a new strategy and a potential drug target for the treatment of inflammatory diseases.
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