Electrical Stimulating Cerebellar Fastigial Nucleus Protects Retina against Ischemia/reperfusion Injury in Rats
Objective To investigate the protective effect and mechanism of electrical stimulating cerebellar fastigial nucleus on retina against ischemia/reperfusion injury in rats. Methods Rats were divided randomly into three groupsrgroupl ( ischemia-reperfusion) , group2 ( electrical-stimulating-treated) , and group3 (sham-operated). Apoptosis of retinal ganglion cells was detected by TUNEL method, and expression of Bcl-2 and Bax were measured by immunohistochemical staining methods. Results Ischemia/reperfusion injury induced the apoptosis of retinal ganglion cells (P<0.05), meanwhile, as ischemia prolonged, the expression of Bcl-2 became weaker, and the expression of Bax became stronger gradually (P<0.05). Electrical stimulating cerebellar fastigial nucleus alleviated these changes in treated group (P <0.05). Conclusions Electrical stimulating cerebellar fastigial nucleus can protect retina against ischemia/reperfusion-injury. The possible mechanism includes upregulating the protein expression of Bcl-2 and downregualting the protein expression of Bax.
国家科技期刊平台
NSTL
万方数据
维普
