首页|GJB4基因在胰腺癌中的表达及其与患者临床病理特征的关系

GJB4基因在胰腺癌中的表达及其与患者临床病理特征的关系

扫码查看
目的 探究GJB4 基因在胰腺癌组织中的表达情况,并研究其与临床病理之间的相关性.方法 运用生物信息学分析癌症基因组图谱(TCGA)和基因型组织表达(GTEx)的RNA测序数据集,对胰腺癌和癌旁中GJB4 mRNA的表达水平进行分析.收集整理 2019 年 1 月至 2023 年 12 月昆明医科大学第二附属医院病理科 120 例胰腺癌和 40 例癌旁样本,采用免疫组化染色方法检测GJB4 蛋白在这两者之间的表达差异.使用RT-qPCR方法检测在 4 个胰腺癌细胞系中GJB4 的表达水平.使用单因素和多因素Cox回归和Kaplan-Meier曲线对GJB4 和胰腺癌患者的临床病理数据进行分析.此外,运用DAVID功能注释生物信息学和GSEA富集分析GJB4 在胰腺癌中的相关通路.结果 GJB4 mRNA的表达水平在胰腺癌中表达高于癌旁组织,且GJB4 的高表达与患者的预后不良差异有统计学意义(P<0.05).免疫组化检测显示,GJB4 蛋白在胰腺癌组织中呈棕黄色颗粒状,主要在细胞质和细胞膜中表达,GJB4 蛋白在胰腺癌中的表达上调(P<0.05).RT-qPCR检测结果显示,在 4 个胰腺癌细胞系中有 3 个胰腺癌细胞系表达上调(P<0.05).COX回归多因素分析发现,GJB4 基因在胰腺癌患者预后生存中扮演了独立的危险因素角色.GO富集分析结果发现GJB4 与胰腺癌跨膜转运、离子通道、信号释放、膜电位调节等过程相关.GSEA分析发现GJB4 在Wnt/β-catenin 信号通路中富集.结论 在胰腺癌组织中,GJB4的高表达水平与患者的临床病理特征密切相关,这可能预示着患者不良预后.GJB4 可能参与调控胰腺癌的Wnt/β-catenin信号通路,并有望成为胰腺癌预后的潜在生物标记物之一.
GJB4 Gene Expression in Relation to Clinical and Pathological Features of Pancreatic Cancer Patients
Objective To investigate the expression of GJB4 gene in pancreatic cancer tissue and its correlation with clinicopathology.Methods The expression levels of GJB4 mRNA in pancreatic cancer and adjacent cancer tissues were analyzed using bioinformatics to analyze the Cancer Genome Atlas(TCGA)and Genotype-Tissue Expression(GTEx)RNA sequencing datasets.A total of 120 pancreatic cancer samples and 40 adjacent cancer samples from the Pathology Department of The Second Affiliated Hospital of Kunming Medical University from January 2019 to December 2023 were collected and sorted.Immunohistochemistry staining method was used to detect the expression difference of GJB4 protein between the two groups.RT-qPCR method was used to detect the expression levels of GJB4 in four pancreatic cancer cell lines.Univariate and multivariate Cox regression and Kaplan-Meier curves were used to analyze the clinical pathological data of GJB4 and pancreatic cancer patients.DAVID functional annotation bioinformatics and GSEA enrichment analysis were used to explore the relevant pathways of GJB4 in pancreatic cancer.Results The expression level of GJB4 mRNA in pancreatic cancer was higher than that in adjacent tissues,and the high expression of GJB4 was significantly associated with poor prognosis of patients(P<0.05).Immunohistochemical analysis showed that GJB4 protein was brown-yellow granular in pancreatic cancer tissues,mainly expressed in cytoplasm and cell membrane,and GJB4 protein expression was up-regulated in pancreatic cancer(P<0.05).The RT-qPCR test results showed that out of 4 pancreatic cancer cell lines,3 of them had upregulated expression(P<0.05).COX regression analysis showed that GJB4 gene was an independent risk factor in the prognosis of pancreatic cancer patients.The results of GO enrichment analysis showed that GJB4 was related to the transmembrane transport,ion channel,signal release and membrane potential regulation of pancreatic cancer.GSEA analysis showed that GJB4 was enriched in the Wnt/β-catenin signaling pathway.Conclusion In pancreatic cancer,the high expression level of GJB4 is closely related to the clinicopathological features of the patients,which may predict the poor prognosis of the patients.GJB4 may be involved in regulating the Wnt/β-catenin signaling pathway of pancreatic cancer,and is expected to be one of the potential biomarkers of pancreatic cancer prognosis.

Pancreatic cancerGJB4Clinicopathological featuresPrognosisBiomarkers

冯润林、邓宗柒、吴梦瑶、王云娜、王瑜、刘桂兰、陶燕萍

展开 >

昆明医科大学第二附属医院病理科,云南 昆明 650101

昆明医科大学第二附属医院检验科,云南 昆明 650101

昆明医科大学第二附属医院消化科,云南 昆明 650101

昆明市第三人民医院急诊科,云南 昆明 650041

展开 >

胰腺癌 GJB4 临床病理特征 预后 生物标记物

2025

昆明医科大学学报
昆明医学院

昆明医科大学学报

影响因子:0.829
ISSN:1003-4706
年,卷(期):2025.46(1)