氢化镁颗粒对牙周致病菌具核梭杆菌的体外抗菌作用
In vitro study on antibacterial effects of magnesium hydride particles against periodontitis-causing Fusobacterium nucleatum
陈雨潇 1饶定成 1隋佰延 1刘昕1
作者信息
- 1. 上海交通大学医学院附属第九人民医院口腔材料科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011
- 折叠
摘要
目的 评价微米氢化镁(MgH2)颗粒对牙周炎致病菌具核梭杆菌的体外抗菌作用,研究可能的抗菌机制.方法 通过扫描电镜(SEM)和X射线衍射仪(XRD)对实验材料MgH2颗粒的尺寸大小、表面形貌和晶相结构进行表征.使用电子顺磁共振技术(EPR)研究其释放行为,同时使用菌落计数实验(CFU)、溴化四甲基偶氮唑盐法(MTT)研究了MgH2颗粒对具核梭杆菌的抗菌效应.结果 MgH2颗粒呈现为尺寸不均一的微米级球状颗粒,平均尺寸为10.52 μm.XRD谱图具有属于MgH2的(110)、(101)、(200)及(211)特征面的衍射信号.在菌落计数实验(CFU)实验中,当材料浓度达到1.5 mg/mL和2 mg/mL时有显著的抑菌效果,抗菌率分别为45.69%和79.24%.在MTT实验中,具核梭杆菌的活性随着MgH2颗粒浓度的增加而逐渐降低,当MgH2颗粒浓度达到2 mg/mL时,其活性接近于零.结论 MgH2颗粒对具核梭杆菌有着良好的抑菌效果,在临床应用方面具有良好的前景.
Abstract
Objective To evaluate the antibacterial effects of micron-sized magnesium hydride(MgH2)particles on periodontitis-causing Fusobacterium nucleatum and to explore the possible antibacterial mechanisms.Methods The size,surface morphology,and crystal phase structure of MgH2 particles were characterized by scan-ning electron microscopy(SEM)and X-ray diffraction(XRD).The release behavior of reactive oxygen radicals from MgH2 particles was studied by electron paramagnetic resonance(EPR)technology,and the antibacterial ef-fects of MgH2 particles on Fusobacterium nucleatum were evaluated by colony counting assay(CFU)and tetrazo-lium bromide salt method(MTT).Results The MgH2 particles presented as micron-sized spherical particles of in-homogeneous size with an average size of 10.52 μm.The XRD spectrum had diffraction signals of the(110),(101),(200),and(211)characteristic planes belonging to MgH2.In the CFU experiment,there was a significant antibacte-rial effect at the MgH2 concentration of 1.5 mg/mL and 2 mg/mL,with antibacterial rates of 45.69%and 79.24%,respectively.In the MTT assay,the viability of Fusobacterium nucleatum decreased gradually with the increase of MgH2 concentration,and its viability was close to zero when the concentration of MgH2 particles reached 2 mg/mL.Conclusion MgH2 particles have good antibacterial effects on Fusobacterium nucleatum,and show promis-ing potential for clinical application.
关键词
氢化镁/抗菌作用/活性氧/具核梭杆菌Key words
Magnesium hydride/Antibacterial effect/Reactive oxygen species/Fusobacterium nucleatum引用本文复制引用
基金项目
国家自然科学基金(82271024)
上海市科委项目(21DZ2291700)
上海市重中之重研究中心项目(2022ZZ01017)
中国医科院医学与健康科技创新工程项目(2019-I2M-5-037)
出版年
2024
NETL
NSTL
万方数据