Effects of fibroblasts derived from different sources on re-epithelialization of keratinocytes
Objective This study aimed to compare the effects of conditioned medium derived from hu-man gingival fibroblasts(hGFs)and dermal fibroblasts(hDFs)on the re-epithelialization of immortalized keratino-cytes(HaCaT cells),explore the critical factors involved in the role of fibroblasts in promoting re-epithelialization,and analyze the possible application of gingival-derived fibroblasts in wound healing.Methods The conditioned medium(CM)of hGFs and hDFs were prepared and used to incubate with HaCaT cells,respectively.The prolifera-tion ability of HaCaT cells was detected by CCK-8 and EdU staining;the cell migration ability was detected by cell scratch assay and Transwell cell migration assay;and a variety of growth factors,cytokines,and chemokines related to wound healing secreted by hGFs and hDFs were quantitatively analyzed by the ABplex custom panel as-say kit and enzyme-linked immunosorbent assay(ELISA).Results The results of CCK-8,EdU staining,cell scratch assay,and Transwell cell migration assay demonstrated a significant enhancement in the proliferation and migration ability of HaCaT cells upon treatment with hGFs-CM and hDFs-CM(P＜0.05).Notably,hGFs-CM ex-hibited a more pronounced effect on promoting HaCaT cell proliferation and migration than hDFs-CM(P＜0.01).There were significant differences in the secreted factors between hGFs and hDFs,with hGFs secreting signifi-cantly more growth factors such as hepatocyte growth factor(HGF)and vascular endothelial growth factor(VEGF)than hDFs.Conclusion The proliferation and migratory ability of keratinocytes were more strongly promoted by the conditioned medium of human gingival-derived fibroblasts than that of human skin-derived fibroblasts,which may be related to differences in the factors produced paracrine.
万方数据
维普
