Local injection of DFO on bone remodeling of alveolar bone:An experimental study in the mouse
Objective:To investigate the effect of local injection of desferrioxamine(DFO)on the bone remodeling of alveolar bone through the regulation of osteoclasts by hypoxia inducible factor-1α(HIF-1α).Methods:DFO was injected locally into the alveolar bone of C57 mice;changes in alveolar bone volume were observed by micro-CT and histological staining;osteoclastogenesis was observed by immunofluorescence and tartrate-resistant acid phosphase(TRAP)staining;the effects of DFO on the differentiation of Raw264.7 to osteoclasts via HIF-1α were observed in vitro by TRAP staining and immunofluorescence.Results:Micro-CT and histological staining showed that local bone density decreased and bone volume decreased after local injection of DFO;histological staining revealed that the number of local osteoclasts increased and HIF-1α expression increased in the DFO injection group compared with the control group;in vitro cell culture revealed that DFO promoted the expression of HIF-1α in Raw264.7 cells and its differentiation to osteoclasts.Conclusion:Local injection of DFO can promote bone remodeling of alveolar bone and differentiation of Raw264.7 into osteoclasts.
osteoclastshypoxia-inducible factor-1αalveolar bone
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