Expression of co-stimulation molecule B7-H4 in oral tongue squamous cell carcinoma and its relationship to prognosis
Objective:To investigate the expression distribution and its relationship to prognosis of co-stimulation molecule B7 homolog4(B7-H4)in oral tongue squamous cell carcinoma(OTSCC).Methods:The study selected paraffin-embedded blocks of cancerous tissues from 99 cases of OTSCC patients and adjacent non-cancerous tissues from 30 OTSCC patients,all of which were archived by the Department of Pathology at the Third Affiliated Hospital of Soochow University between January 2006 and December 2018.Immunohistochemistry was used to examine the B7-H4 expression in cancerous tissue and the para-cancerous tissue.Chi-square test was used to obtain the correlation between the B7-H4 expression in OTSCC tissues and clinical pathological features.Survival analysis(Kaplan-Meier method)was used to analyze the association between B7-H4 expression and patient overall survival(OS).Cox model was used to analyze the correlation between clinical pathological features and patient prognosis.Results:The immunohistochemistry results showed that B7-H4 was not expressed or low intensity expression in 40 cases(40.4% of the total sample),and high intensity expression in 59 cases(59.6%of the total sample)of OTSCC tissues.The results of clinical pathology data analysis in 99 patients with OTSCC showed that the expression of B7-H4 was correlated with clinical stage(P<0.001),and the expression of B7-H4 was correlated with N stage(P<0.001);Kaplan Meier survival analysis showed that the OS of patients with higher B7-H4 expression was lower than that of patients with lower B7-H4 expression,and the difference was statistically significant(HR=1.013,95%CI=1.004-1.022,P<0.05).Uni-factor Cox model analysis indicated that T stage,degree of tissue differentiation,tumor size,and B7-H4 expression were correlated with the prognosis of OTSCC;multi-factor Cox model analysis indicated that B7-H4 high expression(HR=1.015,95%CI=1.005 3-1.024,P<0.01)and degree of tissue differentiation(HR=1.665,95%CI=1.004 7-2.760,P<0.05)were independent prognostic factors of OTSCC patients.Conclusion:B7-H4 expresses highly in OTSCC and can be used as a major factor in the prognosis evaluation of OTSCC patients.
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