索拉非尼抑制口腔鳞状细胞癌细胞的自噬、增殖及迁移
Sorafenib tosylate inhibits autophagy,proliferation and migration of oral squamous cell carcinoma
熊建哲 1张昊 1余伟1
作者信息
- 1. 十堰市人民医院(湖北医药学院附属人民医院)口腔科,十堰 442000
- 折叠
摘要
目的:研究甲苯磺酸索拉非尼(Sorafenib tosylate,ST)对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)CAL-27细胞自噬、增殖、迁移的影响.方法:采用不同浓度的索拉非尼处理CAL-27细胞,使用CCK-8法和细胞克隆形成实验检测其增殖活性;通过细胞划痕实验检测其对CAL-27细胞迁移能力的影响;通过蛋白质印迹法检测Beclin1、LC3B、PCNA的表达.用自噬激活剂雷帕霉素(rapamycin,RA)预处理CAL-27细胞,检测激活自噬后索拉非尼对CAL-27细胞增殖、迁移和Beclin-1、LC3B、PCNA表达的影响.结果:与对照组相比,索拉非尼可显著抑制CAL-27 的细胞增殖和迁移;索拉非尼使Beclin-1和PCNA表达下降、LC3B的胞质形式LC3Ⅰ和LC3B的脂质形式LC3Ⅱ的表达上升;但用RA预处理CAL-27细胞激活自噬后,索拉非尼对CAL-27细胞的增殖、迁移的抑制作用被削弱,Beclin-1和PCNA的表达上升,LC3B的胞质形式LC3 Ⅰ和LC3B的脂质形式LC3Ⅱ的表达下降.结论:索拉非尼可能通过下调Beclin-1抑制自噬,从而抑制CAL-27细胞的增殖和迁移,为OSCC提供潜在的治疗策略.
Abstract
Objective:To study the effect of Sorafenib tosylate(ST)on autophagy,proliferation and migration of CAL-27 cells in oral squamous cell carcinoma(OSCC).Methods:The CAL-27 cells were treated with different concentrations of ST,and their proliferation activity was detected by CCK-8 and cell clone formation assay;the effect of the CAL-27 cells migration was detected by cell wound scratch assay;the expressions of Beclin1,LC3B and PCNA were detected by western blotting.The CAL-27 cells were pretreated with the autophagy activator rapamycin,and the effects of ST on the proliferation,migration and expression of Beclin1,LC3B and PCNA in CAL-27 cells were detected after activation of autophagy.Results:Compared with the control group,ST significantly inhibited the proliferation and migration of the CAL-27 cells.ST decreased the expressions of Beclin-1,PCNA and increased the expressions of the cytoplasmic form LC3Ⅰ and the lipid form LC3Ⅱ of LC3B.However,when the CAL-27 cells were pretreated with RA to activate autophagy,the inhibitory effect of ST on proliferation and migration of the CAL-27 cells were weakened,the expressions of Beclin-1 and PCNA were increased,and the expressions of the cytoplasmic form LC3Ⅰ and the lipid form LC3Ⅱ of LC3B were decreased.Conclusion:ST may inhibit the proliferation and migration of CAL-27 cells by down-regulating Beclin-1,and inhibiting autophagy.Inhibition of autophagy may be a potential therapeutic strategy for OSCC.
关键词
口腔鳞状细胞癌/自噬/增殖/迁移/索拉非尼Key words
oral squamous cell carcinoma/autophagy/proliferation/migration/Sorafenib tosylate引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据