Sorafenib tosylate inhibits autophagy,proliferation and migration of oral squamous cell carcinoma
Objective:To study the effect of Sorafenib tosylate(ST)on autophagy,proliferation and migration of CAL-27 cells in oral squamous cell carcinoma(OSCC).Methods:The CAL-27 cells were treated with different concentrations of ST,and their proliferation activity was detected by CCK-8 and cell clone formation assay;the effect of the CAL-27 cells migration was detected by cell wound scratch assay;the expressions of Beclin1,LC3B and PCNA were detected by western blotting.The CAL-27 cells were pretreated with the autophagy activator rapamycin,and the effects of ST on the proliferation,migration and expression of Beclin1,LC3B and PCNA in CAL-27 cells were detected after activation of autophagy.Results:Compared with the control group,ST significantly inhibited the proliferation and migration of the CAL-27 cells.ST decreased the expressions of Beclin-1,PCNA and increased the expressions of the cytoplasmic form LC3Ⅰ and the lipid form LC3Ⅱ of LC3B.However,when the CAL-27 cells were pretreated with RA to activate autophagy,the inhibitory effect of ST on proliferation and migration of the CAL-27 cells were weakened,the expressions of Beclin-1 and PCNA were increased,and the expressions of the cytoplasmic form LC3Ⅰ and the lipid form LC3Ⅱ of LC3B were decreased.Conclusion:ST may inhibit the proliferation and migration of CAL-27 cells by down-regulating Beclin-1,and inhibiting autophagy.Inhibition of autophagy may be a potential therapeutic strategy for OSCC.
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