Experimental study on the effect of Zfp260 on osteogenic differentiation,proliferation and migration of MC3T3-E1 cells in vitro
Objective:To explore the effect of zinc-finger protein 260(Zfp260)on the osteogenic differentiation,proliferation and migration of mouse embryo osteoblast precursor cells(MC3T3-E1 cells)in vitro.Methods:MC3T3-E1 cells were cultured in vitro and induced to differentiate into osteoblasts.The expression of Zfp260 was detected by real-time quantitative polymerase chain reaction(RT-qPCR)when it was induced for 7 days and 14 days.MC3T3-E1 cells were transfected with siRNA,and the knockdown efficiency of Zfp260 and the expression of alkaline phosphatase(ALP),human bone morphogenetic protein 2(BMP2)and other osteogenic biomarkers in knockdown group and control group were measured by RT-qPCR.The change of migration ability of MC3T3-E1 cells after knockdown of Zfp260 was detected by Transwell,cell scratch assay.The change of proliferation ability of MC3T3-E1 cells after knockdown of Zfp260 was detected by CCK8 experiment.Results:The expression of Zfp260 was significantly up-regulated after inducing osteogenic differentiation of MC3T3-E1 cells in vitro(P<0.05).After using siRNA to knock down Zfp260,the expression of ALP and BMP2 were significantly decreased(P<0.05).Transwell and cell scratch assays showed that the migration ability of MC3T3-E1 cells was inhibited after knocking down Zfp260.CCK8 experiment showed that the proliferation ability of MC3T3-E1 cells increased after knocking down Zfp260.Conclusion:Zfp260 can promote the osteogenic differentiation of MC3T3-E1 cells.
zinc-finger protein 260mouse embryo osteoblast precursor cellsosteogenic differentiation
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