Effect of lincRNA-EPS/miR-24-3p/BMP2 signal axis on enamel development:An experimental study in vitro
Objective:To investigate the effect of long intergenic non-coding RNA-EPS(lincRNA-EPS)/microRNA(miRNA)-24-3p/bone morphogenetic protein 2(BMP2)signal axis on enamel development in vitro.Methods:The overexpressed plasmid of lincRNA-EPS and miR-24-3p mimics were transfected into LS8 cells and their transfection efficiency was detected by real-time quantitative polymerase chain reaction(RT-qPCR).RT-qPCR was used to detect the relative mRNA expression of amelogenesis-related genes(AMELX,AMBN,AMTN,MMP20)and BMP2 after transfection.Western blotting was used to detect the protein expression of BMP2 after transfection.Independent sample t test was used for quantitative comparison between groups.Results:Fluorescence images under microscope and RT-qPCR results showed that lincRNA-EPS overexpression plasmid and miR-24-3p mimics were transfected successfully.The relative mRNA expression of amelogenesis-related genes and BMP2 as well as the protein expression of BMP2 were significantly increased after transfection with lincRNA-EPS overexpression plasmid,while the relative mRNA expression of amelogenesis-related genes and BMP2 as well as the protein expression of BMP2 were significantly decreased after transfection with miR-24-3p mimics.To a certain extent,lincRNA-EPS can inhibit the regulatory effect of miR-24-3p on BMP2,and the difference was statistically significant(P<0.05).Conclusion:Enamel development is regulated by lincRNA-EPS/miR-24-3p/BMP2 signal axis,and lincRNA-EPS can affect the inhibition of miR-24-3p on downstream BMP2 expression,thus promoting enamel development.
long intergenic noncoding RNA-EPSmicroRNA-24-3pbone morphogenetic protein 2enamel development
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