目的:对环状RNA调控大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)及成骨分化的差异表达进行研究,构建大鼠BMSCs全转组录基因数据库,获得特异性基因表达谱,进行功能注释和信号通路等生物信息学分析.方法:采用高通量测序技术检测大鼠BMSCs的成骨分化过程中环状RNA(circular RNA,circRNA)部分的差异性表达.通过基因本体(gene ontology,GO)注释分析以及京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)进行通路富集分析,对具有差异性表达的环状RNA进行功能注释.结果:在大鼠BMSCs成骨分化过程中,每个样本中预测到的circRNA数量为4230个,每个样本中预测到的特异的circRNA为1820个.高通量测序结果显示,在大鼠BMSCs成骨分化过程中共检测到2822个circRNA;总共检测到29个具有差异性表达的circRNA.结论:发现许多差异表达的circRNA与细胞的成骨分化密切相关.这些差异表达circRNA为潜在调控大鼠BMSCs及成骨分化的分子机制提供线索与依据.
Abstract
Objective:To study the differential expression of circular RNAs in the regulation of rat bone marrow mesenchymal stem cells(BMSCs)and osteogenic differentiation,we constructed a database of whole transcriptome genes in rat BMSCs obtained a specific gene expression profile and performed functional annotation and bioinformatics analysis,such as signal pathway analysis.Methods:High-throughput sequencing technology was used to detect the differential expression of circular RNAs(circRNA)during osteogenic differentiation of rat BMSCs.Functional annotation of differentially expressed circRNAs was performed through Gene Ontology(GO)annotation analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results:During osteogenic differentiation process of rat BMSCs,a total of 4,230 circRNAs were predicted in each sample,with 1,820 specific circRNAs predicted in each sample.High-throughput sequencing results showed that 2,822 circRNAs were detected in the osteogenic differentiation process of rat BMSCs,with 29 differentially expressed circRNAs detected.Conclusion:This study revealed that many differentially expressed circRNAs are closely related to cellular osteogenic differentiation.These differentially expressed circRNAs provide clues and evidence for the potential molecular mechanisms regulating rat BMSCs and osteogenic differentiation.
关键词
骨髓间充质干细胞/全转录测序/成骨细胞/基因分析/环状RNA
Key words
bone marrow mesenchymal stem cells/full transcriptional sequencing/osteoblasts/gene analysis/circular RNA
维普
万方数据