Objective:This study aimed to produce alginate gel scaffolds crosslinked with different divalent metal ions(Ca2+,Sr2+,Mg2+and Zn2+)and evaluate their physiochemical and biological properties.Methods:The hydrogels were prepared by reacting sodium alginate solution with mixed ions(Ca2+,Sr2+,Mg2+and Zn2+)as cross-linking agents at a total concentration of 6.5 g/L.The gels were divided into four groups:Ca,Ca-Sr,Ca-Sr-Mg and Ca-Sr-Mg-Zn,with Ca group serving as the control.The physicochemical properties,including surface morphology,degradation rate,ion precipitation concentration and dissolution rate of the hydrogels were investigated.MC3T3-E1 cells were then seeded on the hydrogels to evaluate their effects on cell proliferation and osteogenesis.Results:Compared to the Ca group,the Ca-Sr,Ca-Sr-Mg and Ca-Sr-Mg-Zn groups exhibited more uniformed and dense hydrogel pores,as well as reduced degradation and swelling rates(P＜0.05).The cells adhered to the gel surface also exhibited a change in morphology from spherical to polygonal.The experimental group demonstrated increased cell proliferation,viable cell count,intracellular ALP activity,calcium nodule formation area,and expression of osteogenic genes(Col-1,BMP-2,Runx-2)compared to the Ca group(P＜0.05).The proliferative and osteogenic effects on the gel surface increased sequentially in the Ca-Sr,Ca-Sr-Mg and Ca-Sr-Mg-Zn groups(P＜0.05).Conclusion:The use of a mixture of Ca2+,Sr2+,Mg2+and Zn2+as cross-linking agents improves the physiochemical properties of alginate hydrogels and enhances cell adhesion and osteogenic differentiation of MC3T3-E1 cells on the gel surface compared to single Ca2+cross-linked calcium alginate hydrogels.
关键词
藻酸盐水凝胶/锶、镁、锌/混合金属阳离子/成骨
Key words
alginate hydrogel/strontium,magnesium,zinc/divalent metal ions/osteogenesis
维普
万方数据