Objective:To investigate the therapeutic effect of erbium-chromium-Irdium-scandium gallium garnet(Er,Cr:YSGG)laser therapy on inflammation around microscrews in high glucose environment,and to analyze the relationship with RANK/RANKL signaling pathway.Methods:Twenty normal healthy male New Zealand rabbits were randomly divided into group A(healthy group around microscrews),group B(inflammation group around microscrews)and group C(laser treatment group around microscrews).The diabetes model was established by subcutaneous injection of 2％alloxouracine,and microscrew implants were implanted in mandibular edenceless areas on both sides.Group B and group C were treated with Er,Cr:YSGG laser for inflammation induced at the neck abutment of microscrew implant.The plaque index,probing depth,gingival index and gingival crevicular fluid volume were recorded.The levels of interleukin-1 β(IL-1β),tumor necrosis factor(TNF-α)and interleukin-6(IL-6)in gingival crevicular fluid inflammatory cells were detected by ELISA.CBCT was used to examine the bone resorption status around microscrew implant periinflammation.Western blot analysis of nuclear factor κB(NF-κB)/NF-κB receptor activator(RANK)/NF-κB receptor activator ligand(RANKL)path-related protein expression in gum tissue.Results:CBCT examination showed that compared with group A,the alveolar bone around the microscrew implant in group B had obvious inflammatory absorption,and the microscrew implant-bone interface healing was poor,and bone attachment was insufficient.Compared with group B,there was a small amount of new fiber osteogenesis in one alveolar bone of group C.Compared with group A,group B had higher levels of PI,PD and GI,more gingival crevicular fluid,higher levels of IL-1β,TNF-α and IL-6 in gingival crevicular fluid,and higher expression levels of NF-KBp65,RANK and RANKL in gingival fluid(P＜0.05).Compared with group B,the levels of PI,PD and GI in group C were lower,the amount of gingival crevicular fluid was lower,the levels of IL-1β,TNF-α and IL-6 in gingival crevicular fluid were lower,and the expression levels of NF-κBp65,RANK and RANKL in gingival fluid were lower(P＜0.05).Conclusion:Er,Cr:YSGG laser is effective in the treatment of periarthritis of diabetic rabbit jaw microscrew implant,and may play a role in the regulation of NF-κB/RANK/RANKL pathway expression.
维普
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