Objective:To investigate the antibacterial activity and cytocompatibility of graphene oxide coating on titanium surface.Methods:Graphene oxide(GO)was deposited on titanium surface by electrophoresis at concentrations of 20,60,100,and 140 pg/mL,respectively,and the specimens were noted as the experimental groups,while titanium as the control group.Chemical composition,mechanical and bonding strength of the coatings were evaluated.Antibacterial rates were detected for the assessment of the antibacterial activity of the coatings against Streptococcus mutans(S.mutans)and Porphyromonas gingivalis(P.gingivalis).The effect of the coatings on the proliferation of human gingival fibroblasts(HGFs)was evaluated by CCK-8 assay.Results:After electrophoretic deposition,uniform brown-yellow films were formed on the surfaces of titanium,and the color deepened with the increase of concentration.Raman spectra showed that the characteristic peaks of GO were detected in all experimental groups.The Young's modulus and Vickers hardness of the control group were the highest,while the values of the experimental groups increased with increasing GO concentration,but there were no statistical differences.The scratch curve showed that the turning point occurred first in G140 group and last in G100 group.The antibacterial rate of all groups against S.mutans and P.gingivalis increased with the increase of GO concentration.CCK-8 assay showed that the cell proliferation rate of G140 group was significantly lower than that of the control group.Conclusion:GO coating can be successfully prepared on titanium surface by electrophoresis deposition.When the preparation concentration was 100 μg/mL,GO coating demonstrated excellent antibacterial activity against S.mutans and P.gingivalis,with no obvious cytotoxicity to HGFs.
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