目的:全面地分析高效液相色谱法测定保健食品中维生素 A 含量的不确定度来源和影响不确定度的因素。方法:根据检测过程,建立数学模型,分析影响不确定度因素的来源,通过对不确定度进行量化分析,得出扩展不确定度。结果:高效液相色谱法测定保健食品中维生素 A 含量的扩展不确定度为1.843%。结论:高效液相色谱法测定保健食品中维生素 A 含量的不确定度主要由标准溶液浓度校正、标准溶液的配制和方法总重复性引入。
Objective:Comprehensive to analyze the sources and influencing factors of content uncertainty of Vitamin A in health food by HPLC.Methods:According to the testing process,a mathematical model was established to analyze the sources and influencing factors in uncertainty,through the quantitative analysis of uncertainty,obtained the expanded uncertainty.Result:The expanded uncertainty of Vitamin A in health food by HPLC was 1.843%.Conclusion:The main sources of uncertainty of Vitamin A in health food by HPLC was regulating the concentration of standard solution,preparation of standard solution and the repeatability of method.