首页|SCFP, a novel fiber-specific promoter in cotton
SCFP, a novel fiber-specific promoter in cotton
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To investigate the expression pattern of GhSCFP which was isolated from cotton fiber cDNA library, a 1006 bp upstream fragment of the gene was cloned by chromosome walking and fused to GUSand GFP respectively. Histochemical GUS and GFP fluorescence analysis revealed that the expression of the report genes driven by the promoter sequence was detectable only in outer layer cells during the seed development in the transgentic tobaccos. In transgenic cotton, strong GUS activity was observed in spherical protrusions on 0 dps (days post anthesis) ovule surface, and in the 2-36 dpa fiber cells, while no GUS signals were detected in the root, leaves, stem, corolla, anther and stigma. Our data demon-strated that GhSCFP upstream sequence is a cotton fiber-specific promoter and this promoter will be useful in the molecular research on fiber cell development and in cotton fiber improvements by genetic modification.
HOU Lei、LIU Hao、LI JiaBao、YANG Xia、XIAO YueHua、LUO Ming、SONG ShuiQing、YANG GuangWei、PEI Yan
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Key Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture, Biotechnology Research Center,Southwest University, Chongqing 400716, China
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