Abstract
Rice(Oryza sativa L.)production is threatened by global warming associated with extreme high temper-atures,and rice heat sensitivity is differed when stress occurs between daytime and nighttime.However,the underlying molecular mechanism are largely unknown.We show here that two glycine-rich RNA binding proteins,OsGRP3 and OsGRP162,are required for thermotolerance in rice,especially at night-time.The rhythmic expression of OsGRP3/OsGRP162 peaks at midnight,and at these coincident times,is increased by heat stress.This is largely dependent on the evening complex component OsELF3-2.We next found that the double mutant of OsGRP3/OsGRP162 is strikingly more sensitive to heat stress in terms of survival rate and seed setting rate when comparing to the wild-type plants.Interestingly,the defect in thermotolerance is more evident when heat stress occurred in nighttime than that in day-time.Upon heat stress,the double mutant of OsGRP3/OsGRP162 displays globally reduced expression of heat-stress responsive genes,and increases of mRNA alternative splicing dominated by exon-skipping.This study thus reveals the important role of OsGRP3/OsGRP162 in thermotolerance in rice,and unravels the mechanism on how OsGRP3/OsGRP162 regulate thermotolerance in a diurnal manner.
基金项目
State Key Project of Research and Development Plan of China(2022YFF1001603)
State Key Project of Research and Development Plan of China(2021YFF1000404)
Natural Science Foundation of Zhejiang,China(LD21C020001)
万方数据