Diurnal regulation of alternative splicing associated with thermotolerance in rice by two glycine-rich RNA-binding proteins

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外文摘要：Rice(Oryza sativa L.)production is threatened by global warming associated with extreme high temper-atures,and rice heat sensitivity is differed when stress occurs between daytime and nighttime.However,the underlying molecular mechanism are largely unknown.We show here that two glycine-rich RNA binding proteins,OsGRP3 and OsGRP162,are required for thermotolerance in rice,especially at night-time.The rhythmic expression of OsGRP3/OsGRP162 peaks at midnight,and at these coincident times,is increased by heat stress.This is largely dependent on the evening complex component OsELF3-2.We next found that the double mutant of OsGRP3/OsGRP162 is strikingly more sensitive to heat stress in terms of survival rate and seed setting rate when comparing to the wild-type plants.Interestingly,the defect in thermotolerance is more evident when heat stress occurred in nighttime than that in day-time.Upon heat stress,the double mutant of OsGRP3/OsGRP162 displays globally reduced expression of heat-stress responsive genes,and increases of mRNA alternative splicing dominated by exon-skipping.This study thus reveals the important role of OsGRP3/OsGRP162 in thermotolerance in rice,and unravels the mechanism on how OsGRP3/OsGRP162 regulate thermotolerance in a diurnal manner.

外文关键词：

Alternative splicingDiurnal regulationGlycine-rich proteinOryza sativa L.Thermotolerance

作者：

Chuang Yang、Anni Luo、Hai-Ping Lu、Seth Jon Davis、Jian-Xiang Liu

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作者单位：

State Key Laboratory of Plant Environmental Resilience,College of Life Sciences,Zhejiang University,Hangzhou 310027,China

Department of Biology,University of York,York YO105DD,UK

基金：

State Key Project of Research and Development Plan of ChinaState Key Project of Research and Development Plan of ChinaNatural Science Foundation of Zhejiang,China

项目编号：

2022YFF10016032021YFF1000404LD21C020001

出版年：

2024

DOI：

10.1016/j.scib.2023.11.046

科学通报(英文版)

中国科学院

科学通报(英文版)

CSTPCD

ISSN：1001-6538

年,卷(期)：2024.69(1)

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