首页|In vivo production of CAR-T cells using virus-mimetic fusogenic nanovesicles
In vivo production of CAR-T cells using virus-mimetic fusogenic nanovesicles
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Engineered T cells expressing chimeric antigen receptor(CAR)exhibit high response rates in B-cell malig-nancy treatments and possess therapeutic potentials against various diseases.However,the complicated ex vivo production process of CAR-T cells limits their application.Herein,we use virus-mimetic fusogenic nanovesicles(FuNVs)to produce CAR-T cells in vivo via membrane fusion-mediated CAR protein delivery.Briefly,the FuNVs are modified using T-cell fusogen,adapted from measles virus or reovirus fusogens via displaying anti-CD3 single-chain variable fragment.The FuNVs can efficiently fuse with the T-cell mem-brane in vivo,thereby delivering the loaded anti-CD19(αCD19)CAR protein onto T-cells to produceαCD19 CAR-T cells.These αCD19 CAR-T cells alone or in combination with anti-OX40 antibodies can treat B-cell lymphoma without inducing cytokine release syndrome.Thus,our strategy provides a novel method for engineering T cells into CAR-T cells in vivo and can further be employed to deliver other ther-apeutic membrane proteins.
CAR-TNanovesicleFusogenCancer immunotherapy
Gui Zhao、Yue Zhang、Cong-Fei Xu、Jun Wang
展开 >
School of Biomedical Sciences and Engineering,South China University of Technology,Guangzhou International Campus,Guangzhou 511442,China
National Engineering Research Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou 510006,China
Guangdong Provincial Key Laboratory of Biomedical Engineering,South China University of Technology,Guangzhou 510006,China
Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education,South China University of Technology,Guangzhou 510006,China
展开 >
National Key R&D Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaGuangdong Basic and Applied Basic Research FoundationScience and Technology Program of Guangzhou,ChinaFundamental Research Funds for the Central Universities
NETL
NSTL
万方数据
