首页|Three-dimensional,dual-color nanoscopy enabled by migrating photon avalanches with one single low-power CW beam

Three-dimensional,dual-color nanoscopy enabled by migrating photon avalanches with one single low-power CW beam

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The development of super-resolution fluorescence microscopy is very essential for understanding the physical and biological fundamentals at nanometer scale.However,to date most super-resolution modal-ities require either complicated/costly purpose-built systems such as multiple-beam architectures or complex post-processing procedures with intrinsic artifacts.Achieving three-dimensional(3D)or multi-channel sub-diffraction microscopic imaging using a simple method remains a challenging and struggling task.Herein,we proposed 3D highly-nonlinear super-resolution microscopy using a single-beam excitation strategy,and the microscopy principle was modelled and studied based on the ultrahigh nonlinearity enabled by photon avalanches.According to the simulation,the point spread function of highly nonlinear microscopy is switchable among different modes and can shrink three-dimensionally to sub-diffraction scale at the photon avalanche mode,Experimentally,we demonstrated 3D optical nanoscopy assisted with huge optical nonlinearities in a simple laser scanning configuration,achieving a lateral resolution down to 58 nm(λ/14)and an axial resolution down to 185 nm(λ/5)with one single beam of low-power,continuous-wave,near-infrared laser.We further extended the photon avalanche effect to many other emitters to develop multi-color photon avalanching nanoprobes based on migrating photon avalanche mechanism,which enables us to implement single-beam dual-color sub-diffraction super-resolution microscopic imaging.

Super-resolution microscopyNonlinear opticsUpconversion nanoparticlesPhoton avalanches

Zhimin Zhu、Yusen Liang、Qi Zhao、Hui Wu、Binxiong Pan、Shuqian Qiao、Baoju Wang、Qiuqiang Zhan

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Centre for Optical and Electromagnetic Research,Guangdong Engineering Research Centre of Optoelectronic Intelligent Information Perception,South China Academy of Advanced Optoelectronics,South China Normal University,Guangzhou 510006,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaGuangdong Basic and Applied Basic Research FoundationGuangdong Basic and Applied Basic Research FoundationGuangdong Basic and Applied Basic Research FoundationGuangdong Provincial Science Fund for Distinguished Yong ScholarsGuangzhou Basic and Applied Basic Research FoundationChina Postdoctoral Science FoundationChina Postdoctoral Science Foundation

623350086212202862105106119741232023B15150400182022A15150113952019A0505100372018B0303060152022010103762023T1602372021M691089

2024

10.1016/j.scib.2023.12.042

科学通报(英文版)
中国科学院

科学通报(英文版)

CSTPCD
ISSN：1001-6538
年,卷(期)：2024.69(4)
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