Three-dimensional,dual-color nanoscopy enabled by migrating photon avalanches with one single low-power CW beam

Zhimin Zhu ¹Yusen Liang ¹Qi Zhao ¹Hui Wu ¹Binxiong Pan ¹Shuqian Qiao ¹Baoju Wang ¹Qiuqiang Zhan¹

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作者信息

1. Centre for Optical and Electromagnetic Research,Guangdong Engineering Research Centre of Optoelectronic Intelligent Information Perception,South China Academy of Advanced Optoelectronics,South China Normal University,Guangzhou 510006,China
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Abstract

The development of super-resolution fluorescence microscopy is very essential for understanding the physical and biological fundamentals at nanometer scale.However,to date most super-resolution modal-ities require either complicated/costly purpose-built systems such as multiple-beam architectures or complex post-processing procedures with intrinsic artifacts.Achieving three-dimensional(3D)or multi-channel sub-diffraction microscopic imaging using a simple method remains a challenging and struggling task.Herein,we proposed 3D highly-nonlinear super-resolution microscopy using a single-beam excitation strategy,and the microscopy principle was modelled and studied based on the ultrahigh nonlinearity enabled by photon avalanches.According to the simulation,the point spread function of highly nonlinear microscopy is switchable among different modes and can shrink three-dimensionally to sub-diffraction scale at the photon avalanche mode,Experimentally,we demonstrated 3D optical nanoscopy assisted with huge optical nonlinearities in a simple laser scanning configuration,achieving a lateral resolution down to 58 nm(λ/14)and an axial resolution down to 185 nm(λ/5)with one single beam of low-power,continuous-wave,near-infrared laser.We further extended the photon avalanche effect to many other emitters to develop multi-color photon avalanching nanoprobes based on migrating photon avalanche mechanism,which enables us to implement single-beam dual-color sub-diffraction super-resolution microscopic imaging.

Key words

Super-resolution microscopy/Nonlinear optics/Upconversion nanoparticles/Photon avalanches

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基金项目

National Natural Science Foundation of China(62335008)

National Natural Science Foundation of China(62122028)

National Natural Science Foundation of China(62105106)

National Natural Science Foundation of China(11974123)

Guangdong Basic and Applied Basic Research Foundation(2023B1515040018)

Guangdong Basic and Applied Basic Research Foundation(2022A1515011395)

Guangdong Basic and Applied Basic Research Foundation(2019A050510037)

Guangdong Provincial Science Fund for Distinguished Yong Scholars(2018B030306015)

Guangzhou Basic and Applied Basic Research Foundation(202201010376)

China Postdoctoral Science Foundation(2023T160237)

China Postdoctoral Science Foundation(2021M691089)

出版年

2024

科学通报(英文版)

中国科学院

科学通报(英文版)

CSTPCD

ISSN：1001-6538

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