首页|Quantitative single-virus tracking for revealing the dynamics of SARS-CoV-2 fusion with plasma membrane

Quantitative single-virus tracking for revealing the dynamics of SARS-CoV-2 fusion with plasma membrane

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Viral envelope fusion with the host plasma membrane(PM)for genome release is a hallmark step in the life cycle of many enveloped viruses.This process is regulated by a complex network of biomolecules on the PM,but robust tools to precisely elucidate the dynamic mechanisms of virus-PM fusion events are still lacking.Here,we developed a quantitative single-virus tracking approach based on highly efficient dual-color labelling of viruses and batch trajectory analysis to achieve the spatiotemporal quantification of fusion events.This approach allows us to comprehensively analyze the membrane fusion mechanism utilized by pseudotyped severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)at the single-virus level and precisely elucidate how the relevant biomolecules synergistically regulate the fusion pro-cess.Our results revealed that SARS-CoV-2 may promote the formation of supersaturated clusters of cholesterol to facilitate the initiation of the membrane fusion process and accelerate the viral genome release.

SARS-CoV-2Fluorescence labellingMembrane fusionQuantum dotSingle-virus tracking

Hao-Yang Liu、Yusi Hu、Cong Yu、Zhi-Gang Wang、Shu-Lin Liu、Dai-Wen Pang

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State Key Laboratory of Medicinal Chemical Biology,Frontiers Science Center for New Organic Matter,Frontiers Science Center for Cell Responses,Tianjin Key Laboratory of Biosensing and Molecular Recognition,Research Center for Analytical Sciences,College of Chemistry,and School of Medicine,Nankai University,Tianjin 300071,China

Haihe Laboratory of Sustainable Chemical Transformations,Tianjin 300192,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaFundamental Research Funds for the Central Universities of China

2229303222293030918591232019YFA021050063211023

2024

10.1016/j.scib.2023.11.020

科学通报(英文版)
中国科学院

科学通报(英文版)

CSTPCD
ISSN：1001-6538
年,卷(期)：2024.69(4)
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