Analysis of the influence of mixing and resting time on the results of routine blood samples before examination
Objective To explore the effect of mixing and resting time on the test results of blood routine (CBC) specimens. Methods The patients who underwent physical examination in our hospital from July 2021 to June 2022 were selected as the research objects. Peripheral venous blood samples were collected for CBC testing. Before the blood samples were examined, the samples were left for a period of time after anticoagulation treatment, and the patients were grouped with the resting time as the variable. The results were compared after 15 min, 30 min, 1 h, 2 h, 3 h, 5 h, 7 h, 9 h and 11 h CBC test results of stationary blood samples -- red blood cell count (RBC), platelet count (PLT), hemoglobin (Hb), hemoglobin hematocrit (HCT), white blood cell count (WBC), neutrophils (Neu), lymphocytes (Lym). Results There was no statistical significance in RBC, Hb, HCT and Lym indexes of blood samples at different time periods (P＞0.05), but PLT indexes showed a significant downward trend with the increase of resting time, while WBC and Neu indexes showed an increasing trend, with significant statistical significance (P＜0.05). The indexes of PLT, WBC and Neu all changed significantly during 2 ～ 3 h. Conclusion Through this study, it is found that the extension of mixing and standing time will lead to differences in test results to some extent, and the influence on RBC, Hb, HCT and Lym indexes is small, but the influence on PLT, WBC and Neu indexes is more obvious. Therefore, medical personnel should control the testing time in the actual work, avoid immediate testing, and also avoid testing blood samples 2 hours later, and try to control the testing time between 0.5 and 2 hours, so as to improve the accuracy of testing.
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