目的 通过慢性高氧诱导建立支气管肺发育不良(BPD)动物模型，研究慢性高氧对肺功能的影响。方法 将 C57BL/6J 新生24h内的小鼠暴露在85%的氧气中，用 HE 染色观察小鼠肺组织的形态学变化，检测小鼠的特殊气道阻力(sRaw)，潮气量(TV)，每分钟通气量(MV)，呼吸频率(F)等肺功能指标。结果 高氧组小鼠与对照组相比，体重下降，病死率上升，肺泡间隔增厚、破裂，肺泡腔扩大，炎性浸润，肺泡平均截距增高，放射肺泡数量下降，肺部发育受阻。高氧组小鼠的呼吸频率、潮气量、肺通气量在暴露于慢性高氧后明显下降，且特异性气道阻力增加，差异具有统计学意义(P<0。05)。结论 慢性高氧症增加小鼠气道阻力，损害肺功能。
The Study of Effect of Chronic Hyperoxia on Mouse Pulmonary Function
Objective To establish a bronchopulmonary dysplasia(BPD)animal model by chronic hyperoxia exposure and to investigate the effects of chronic hyperoxia on pulmonary function.Methods C57BL/6J neonatal mice were exposed to 85%oxygen for 24 hours from 24 hours after birth.HE staining was used to observe the morphological changes of lung tissue in mice.Special airway resistance(sRaw),tidal volume(TV),minute ventilation(MV),respiratory frequency(F),and other pulmonary function indicators were measured.Results Compared with the control group,mice in the hyperoxia group had lower body weight,higher mortality rate,thickened and ruptured alveolar septa,enlarged alveolar cavities,inflammatory infiltration,increased average alveolar intercept,decreased number of alveoli,and arrested lung development.The respiratory frequency,tidal volume,and pulmonary ventilation volume of mice in the hyperoxia group decreased significantly after exposure to chronic hyperoxia,and specific airway resistance increased,with statistical significance(P<0.05).Conclusion Chronic hyperoxia increases airway resistance and impairs pulmonary function in mice.
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