To further investigate the application of methane oxidizing mixed bacteria in coal seam gas treatment,a survey was conducted on the highly active methane oxidizing mixed bacteria in nature.At the same time,the growth characteristics of methane oxidizing mixed bacteria and the variation of methane oxidation efficiency under different pH environments were studied,and then the dynamic contact characteristics of the bacterial solution on the surface of coal samples were compared and analyzed.The results show that the OD600 value of the bacterial solution increases by about 19 times during the cultivation period when pH=6.75,and 47.3 mL of methane gas is consumed,achieving the strongest growth ability and methane oxidation efficiency.The acid-base property could inhibit the growth of microorganisms,and the mixed bacteria could regulate the environmental acidity and alkalinity through their own metabolism,promoting the pH value of the bacterial solution to change in the direction of suitable growth.Compared with the bacterial solution with pH=6.75,the initial contact angle and equilibrium contact angle of the remaining groups increase by a maximum of 21.40％and 48.64％,indicating that a suitable pH environment can enhance microbial activity,promote its adsorption on coal samples,improve the contact ability of bacterial solution and coal samples.The research results can provide a theoretical support for the microbial technology in coalbed methane treatment.
关键词
甲烷氧化混合菌/瓦斯治理/生长特性/接触能力/氧化规律
Key words
Methane oxidation mixed bacteria/Gas control/Growth characteristics/Contact ability/Oxidation law
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