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不同pH条件下的甲烷氧化混合菌生长特性及效能研究

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为深入研究甲烷氧化混合菌群在煤层瓦斯治理方面的应用,对自然界中具有较强活性的甲烷氧化混合菌群进行了调查研究,同时对不同pH环境下甲烷氧化混合菌的生长特性及甲烷氧化效能的变化规律进行了研究,进而对比分析了菌液在煤样表面的动态接触特征.结果表明:培养周期内pH为6.75的条件下,菌液OD600值增大了约19倍,消耗甲烷气体47.3 mL,生长能力和甲烷氧化效能最强;酸碱度可抑制微生物的生长,混合菌群可通过自身代谢调节环境酸碱性,促使菌液pH向适宜生长的方向变化;相较于pH为6.75的菌液,其余各组的初始接触角与平衡接触角最高增大了21.40%和48.64%,表明适宜pH环境可提高微生物活性,促进其在煤样表面吸附,提高菌液与煤样的接触能力.研究成果为微生物技术在煤层瓦斯治理提供理论支撑.
Growth Characteristics and Efficiency of Methane Oxidation Mixed Bacteria Under Different pH Conditions
To further investigate the application of methane oxidizing mixed bacteria in coal seam gas treatment,a survey was conducted on the highly active methane oxidizing mixed bacteria in nature.At the same time,the growth characteristics of methane oxidizing mixed bacteria and the variation of methane oxidation efficiency under different pH environments were studied,and then the dynamic contact characteristics of the bacterial solution on the surface of coal samples were compared and analyzed.The results show that the OD600 value of the bacterial solution increases by about 19 times during the cultivation period when pH=6.75,and 47.3 mL of methane gas is consumed,achieving the strongest growth ability and methane oxidation efficiency.The acid-base property could inhibit the growth of microorganisms,and the mixed bacteria could regulate the environmental acidity and alkalinity through their own metabolism,promoting the pH value of the bacterial solution to change in the direction of suitable growth.Compared with the bacterial solution with pH=6.75,the initial contact angle and equilibrium contact angle of the remaining groups increase by a maximum of 21.40%and 48.64%,indicating that a suitable pH environment can enhance microbial activity,promote its adsorption on coal samples,improve the contact ability of bacterial solution and coal samples.The research results can provide a theoretical support for the microbial technology in coalbed methane treatment.

Methane oxidation mixed bacteriaGas controlGrowth characteristicsContact abilityOxidation law

赵双、张瑞林、王思琪、周银波、田坤云、宫伟东

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河南理工大学能源科学与工程学院,河南焦作市 454003

河南工程学院资源与安全工程学院,河南郑州4511913

中国石化销售股份有限公司河南周口石油分公司,河南周口市 466000

甲烷氧化混合菌 瓦斯治理 生长特性 接触能力 氧化规律

国家自然科学基金项目河南省高校科技创新团队计划和创新人才支持计划项目河南省高校科技创新团队计划和创新人才支持计划项目河南省科协青年人才托举工程项目河南省科技攻关和高校重点科研项目河南省科技攻关和高校重点科研项目河南省科技攻关和高校重点科研项目河南省科技攻关和高校重点科研项目

52174168221RTSTHN00924HASTIT0192023HYTP01422210232020822210232046623A62000424A440002

2024

矿业研究与开发
长沙矿山研究院有限责任公司 中国有色金属学会

矿业研究与开发

CSTPCD北大核心
影响因子:0.763
ISSN:1005-2763
年,卷(期):2024.44(9)