不同消毒液对寒地早粳稻花药离体培养结果的影响
Effects of Different Disinfectants on the Results of in Vitro Culture of Early Japonica Rice Anther in Cold Area
刘宇 1姜新龙 2姜玉伟 2陆文静 3刘辉 3张献国 3庞欢欢 2张海建2
作者信息
- 1. 黑龙江农业职业技术学院,黑龙江 佳木斯 154007
- 2. 佳木斯龙粳种业有限公司,黑龙江 佳木斯 154026
- 3. 黑龙江省农业科学院 水稻研究所,黑龙江 佳木斯 154025
- 折叠
摘要
花药离体培养培育的植株是纯合体,可以在早世代选出所期望的性状并稳定遗传,使育种年限降低.花药离体培养操作过程中,消毒处理很重要.试验选用不同消毒液对F1 代植株进行处理,结果表明:84 消毒液和新洁尔灭都对F1 代植株起到了一定的消毒作用,花药污染率分别为 6.67%和 7.78%,愈伤诱导率超过 50%,均正常分化出绿苗.
Abstract
The plants cultivated by anther in vitro culture are homozygotes,which can select the desired traits in early generation and inherit them stably,thus reducing the breeding life.Disinfection is very important in the process of anther culture in vitro.In the experiment,different disinfectants were used to treat F1 generation plants.The results showed that both the 84 disinfectant and the Xinjieermie had certain disinfection effect on F1 generation plants,the anther contamination rate was 6.67%and 7.78%respectively,the callus induction rate was more than 50%,and green seedlings were differentiated normally.
关键词
消毒液/花药离体培养/水稻Key words
Disinfectant/Anther culture in vitro/Rice引用本文复制引用
出版年
2024
NETL
NSTL
万方数据