Effects of Different Disinfectants on the Results of in Vitro Culture of Early Japonica Rice Anther in Cold Area
The plants cultivated by anther in vitro culture are homozygotes,which can select the desired traits in early generation and inherit them stably,thus reducing the breeding life.Disinfection is very important in the process of anther culture in vitro.In the experiment,different disinfectants were used to treat F1 generation plants.The results showed that both the 84 disinfectant and the Xinjieermie had certain disinfection effect on F1 generation plants,the anther contamination rate was 6.67%and 7.78%respectively,the callus induction rate was more than 50%,and green seedlings were differentiated normally.
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