HPA和MMP9对非小细胞肺癌患者临床预后评价的意义研究
Significance of serum heparanase and matrix metalloproteinases 9 in the prognosis of non-small cell lung cancer
夏彦东 1常延河 1杨宏秀 1刘丽1
作者信息
- 1. 063000 河北 唐山，唐山市开滦总医院检验科
- 折叠
摘要
目的：检测非小细胞肺癌( NSCLC)患者血清HPA和MMP9水平，分析其与NSCLC患者淋巴结转移以及预后的关系。方法选取80例NSCLC患者和30例健康对照，采用酶联免疫法检测HPA和MMP9的血清水平，分析HPA和MMP9与患者临床病理特征的关系，通过受试者工作曲线分析血清HPA和MMP9用于判断NSCLC患者是否发生淋巴结转移的可行性，通过Kaplan-Meier法进行分析HPA和MMP9预测病人预后的临床意义。结果 NSCLC患者血清HPA和MMP9值均高于健康对照组；血清HPA与淋巴结转移、远处转移有关，血清MMP9值与TNM分期、淋巴结转移、远处转移有关；血清HPA和MMP9用于预测NSCLC患者淋巴结转移情况的曲线下面积分别为0．732和0．785；NSCLC患者血清HPA和MMP9越高，患者预后越差。结论血清HPA和MMP9均可用于确定NSCLC患者是否发生淋巴结转移，并可用于评估病人预后。
Abstract
Objective To detect the level of serum heparanase ( HPA ) and matrix metalloproteinases 9 (MMP9) in non-small cell lung cancer (NSCLC) patients, and to investigate the relationship of the two indexes with lymph node metastasis and prognosis. Methods The serum levels of HPA and MMP9 were detected in 80 NSCLC patients and 30 health controls by enzyme-linked immunosorbent assay ( ELISA) . The clinical and pathologic features were analyzed through the levels of HPA and MMP9. The specificity and sensitivity of serum HPA and MMP9 to de-termine lymph node metastasis or not were analyzed by receiver operating characteristic ( ROC) curve. Kaplan-Meier method was used to evaluate overall survival according to cutoff serum levels. Results The serum levels of HPA and MMP9 were higher in NSCLC patients than in health controls. The serum level of HPA was associations with lymph node metastasis and distance metastasis. The serum level of MMP9 was associated with TNM stage, lymph node me-tastasis and distance metastasis. The area under the curve ( AUC) was 0. 732 and 0. 785 respectively for identifying lymph node metastases in patients with serum HPA and MMP9. NSCLC patients with higher levels of serum HPA and MMP9 had poorer prognosis. Conclusion The serum levels of HPA and MMP9 may be helpful biomarkers in identif-ying lymph node metastases, which can also be useful for evaluating their prognosis.
关键词
非小细胞肺癌/淋巴结转移/乙酰肝素酶/基质金属蛋白酶9/预后Key words
non-small cell lung cancer/lymph node metastasis/heparanase/matrix metalloproteinases 9/prognosis引用本文复制引用
出版年
2015
NETL
NSTL
维普
万方数据