The expression and early efficacy prediction value of miRNA-99b,LL-37,and IP-10 in multidrug-resistant pulmonary tuberculosis patients
Objective To analyze the expression of micro RNA-99B(miRNA-99b),antimicrobial peptide LL-37,interferon-gamma-induced protein 10(IP-10)in patients with multidrug-resistant pulmonary tuberculosis(MDR-PTB)and the value of early curative effect prediction.Methods A total of 108 MDR-PTB patients admit-ted to the hospital from November 2022 to February 2024 were classified as MDR-PTB group and were divided into mild group(n=71)and moderate to severe group(n=37)by Bandim TBscore.Eighty-seven patients with non-drug-resistant tuberculosis(DS-TB)were included in the DS-TB group and 90 healthy volunteers were included in the healthy control group.The expression levels of miRNA-99b,LL-37,and IP-10 between the groups were detected and evaluated,and the clinical efficacy after 8 weeks of treatment was also evaluated.Pearson was used to evaluate the correlation between the expression levels of miRNA-99b,LL-37,and IP-10 and the clinical efficacy of MDR-PTB patients.The predictive value of indicators was evaluated by multivariate Logistic regression,and the predictive value of the ROC curve analysis model was performed.Results The miRNA-99b,LL-37,and IP-10 levels in the MDR-PTB group were higher than those in the DS-TB group and healthy control group(P＜0.05),and the miRNA-99b,LL-37,and IP-10 levels in the DS-TB group were higher than those in the healthy control group(P＜0.05);In the MDR-PTB group,the expression levels of miRNA-99b,LL-37,and IP-10 in the moderate to the severe group were higher than those in the mild group(P＜0.05);After 4 weeks of treatment and 8 weeks of treatment,the miRNA-99b,LL-37,and IP-10 levels in MDR-PTB patients were lower than before treatment(P＜0.05);After 8 weeks of treatment,the clinical effective rate of 108 MDR-PTB patients was 88.89％;MiRNA-99b,LL-37,and IP-10 are all negatively correlated with efficacy(r=-0.693,-0.706,-0.497,P＜0.05);High levels of expression of miR-NA-99b,LL-37,and IP-10 were independent risk factors for the occurrence and development of MDR-PTB,with sta-tistical significance(P＜0.05).ROC curve analysis showed that the combined detection of miRNA-99b,LL-37,and IP-10 had an AUC of 0.937(95％CI:0.863-0.974)(Z=7.691,7.069,8.068,P＜0.001),which was higher than that of a single detection result,and the combined detection had the highest sensitivity(96.91％)and specificity(96.34％).Conclusion High levels of miRNA-99b,LL-37,and IP-10 expression are involved in the occurrence and development of MDR-PTB,and the combination of these three factors has high application value in early efficacy prediction of MDR-PTB patients.
miRNA-99bLL-37IP-10Multidrug resistant pulmonary tuberculosisEarly efficacypre-dictive value
万方数据
维普
